EurekaMag
+ Translate
+ Most Popular
Gaucher's disease;thirty-two years experience at Siriraj Hospital
A study of Macrobathra Meyrick from China (Lepidoptera, Cosmopterigidae)
First occurrence in ores of tetragonal chalcocite
Effects of trace element nutrition on sleep patterns in adult women
N.Z. range management guidelines. 2. Design of grazing management systems for tussock country
A case of lipoma of the esophagus
A revision of world Acanthosomatidae (Heteroptera: Pentatomidae): keys to and descriptions of subfamilies, tribes and genera, with designation of types
Life history of the coronate scyphozoan Linuche unguiculata (Swartz, 1788)
Perceptual restoration of obliterated sounds
Mutagenicity studies on two chromium(III) coordination compounds
The formation of the skeleton. I. Growth of a long bone. 1st appearance of a center of calcification
Leucopenia and abnormal liver function in travellers on malaria chemoprophylaxis
The joint commission: four key root causes loom large in sentinel event data
Treatment of vitiligo with topical 15% lactic acid solution in combination with ultra violet-A
Behaviour of dairy cows within three hours after feed supply: I. Influence of housing type and time elapsing after feed supply
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Management and control of patients with type 2 diabetes mellitus in Lebanon: results from the International Diabetes Management Practices Study (IDMPS)
The diet composition and nutritional knowledge of patients with anorexia nervosa
Physoporella croatica Herak, 1958 of the Slovak karst Anisian (Slovakia, the West Carpathians Mts.)
Bright lights, big noise. How effective are vehicle warning systems?
Ein Plesiosaurier-Rest mit Magensteinen aus mittlerem Lias von Quedlinburg
Incidence of Chlamydia trachomatis in patients with sterility
Monster soup: the microscope and Victorian fantasy
Preliminary tests with residual sprays against poultry lice
Duration of the life of plants in phylogeny

Endo-beta-1,4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action


Endo-beta-1,4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action



Journal of Biotechnology 92(3): 267-277



ISSN/ISBN: 0168-1656

PMID: 11689251

DOI: 10.1016/s0168-1656(01)00367-4

Two variants of an endo-beta-1,4-mannanase from the digestive tract of blue mussel, Mytilus edulis, were purified by a combination of immobilized metal ion affinity chromatography, size exclusion chromatography in the absence and presence of guanidine hydrochloride and ion exchange chromatography. The purified enzymes were characterized with regard to enzymatic properties, molecular weight, isoelectric point, amino acid composition and N-terminal sequence. They are monomeric proteins with molecular masses of 39216 and 39265 Da, respectively, as measured by MALDI-TOF mass spectrometry. The isoelectric points of both enzymes were estimated to be around 7.8, however slightly different, by isoelectric focusing in polyacrylamide gel. The enzymes are stable from pH 4.0 to 9.0 and have their maximum activities at a pH about 5.2. The optimum temperature of both enzymes is around 50-55 degrees C. Their stability decreases rapidly when going from 40 to 50 degrees C. The N-terminal sequences (12 residues) were identical for the two variants. They can be completely renatured after denaturation in 6 M guanidine hydrochloride. The enzymes readily degrade the galactomannans from locust bean gum and ivory nut mannan but show no cross-specificity for xylan and carboxymethyl cellulose. There is no binding ability observed towards cellulose and mannan.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011665261

Download citation: RISBibTeXText

Related references

Endo-b-1,4-mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action. Journal of Biotechnology 92(3): 7-77, 2002

Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-beta-1,4-glucanase from blue mussel, Mytilus edulis. European Journal of Biochemistry 267(16): 4970-4977, 2000

Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo-b-1,4-glucanse from blue mussel, Mytilus edulis. European Journal of Biochemistry 267(16): 70-7, 2000

Purification, characterization and amino-acid sequence analysis of a thermostable, low molecular mass endo--1,4-glucanase from blue mussel, Mytilus edulis. FEBS Journal 267(16): 4970-4977, 2000

Cloning and sequencing of a molluscan endo-beta-1,4-glucanase gene from the blue mussel, Mytilus edulis. European Journal of Biochemistry 268(13): 3718-3727, 2001

The initial mode of action of copper on the cardiac physiology of the blue mussel, Mytilus edulis. Aquatic Toxicology 52(1): 29-38, 2001

Purification and characterization of the red carotenoprotein from the muscle of blue mussel, Mytilus edulis. Bulletin of the Korean Fisheries Society 27(5): 482-494, 1994

Three-dimensional crystal structure and enzymic characterization of beta-mannanase Man5A from blue mussel Mytilus edulis. Journal of Molecular Biology 357(5): 1500-1510, 2006

Cloning and sequencing of a molluscan endo--1,4-glucanase gene from the blue mussel, Mytilus edulis. FEBS Journal 268(13): 3718-3727, 2001

Cloning and sequencing of a molluscan endo-b1,4-glucanase gene from the blue mussel, Mytilus edulis. European Journal of Biochemistry 268(13): 18-27, 2001

Characterization of six microsatellite loci in the Baltic blue mussel Mytilus trossulus and cross-species amplification in North Sea Mytilus edulis. Conservation Genetics 9(4): 1003-1005, 2008

Toxoplasma gondii and Other Zoonotic Protozoans in Mediterranean Mussel ( Mytilus galloprovincialis) and Blue Mussel ( Mytilus edulis): A Food Safety Concern?. Journal of Food Protection 82(3): 535-542, 2019

Use of an index based on the blue mussel (Mytilus edulis and Mytilus trossulus) digestive gland weight to assess the nutritional quality of mussel farm sites. Aquaculture 241(1/4): 633-654, 2004

Characterization of ten microsatellite loci in the blue mussel Mytilus edulis. Journal of Shellfish Research 28(3): 547-551, 2009

Comparison between two protocols for the isolation of metallothioneins within the mussel, Mytilus edulis Comparaison de deux protocoles dextraction pour la purification des metallothioneines de la moule Mytilus edulis. Journal de Recherche Oceanographique. Decembre; 224: 151-156, 1997