+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression



A broad competence to respond to SHORT ROOT revealed by tissue-specific ectopic expression



Development 131(12): 2817-2826



In plants, cell fate specification depends primarily on position rather than lineage. Recent results indicate that positional information can be transmitted through intercellular trafficking of transcription factors. The SHORT ROOT (SHR) gene, a member of the GRAS family of putative transcription factors, is involved in root radial patterning in Arabidopsis. Correct radial patterning depends on the positional information transmitted through limited SHR intercellular movement and translated into cell division and specification by competent target cells. To investigate the regulation of SHR movement and the competence to respond to it, we drove expression of a translational fusion SHR::GFP using four different tissue-specific promoters. In a wild-type background, SHR::GFP was not able to move from either phloem companion cells or epidermal cells, both of which have been shown to support movement of other proteins, suggesting a requirement for tissue-specific factors for SHR movement. When expressed from its native promoter in plants with multiple endodermal layers, SHR::GFP was not able to move beyond the first endodermal layer, indicating that movement is not limited by a mechanism that recognizes boundaries between cell types. Surprisingly, movement of SHR::GFP was observed when ectopic expression from an epidermal promoter was placed in a scarecrow (scr) mutant background, revealing a possible role for SCR in limiting movement. Analysis of the competence to respond to SHR-mediated cell specification activity indicated that it was broadly distributed in the epidermal lineage, while competence to respond to the cell division activity of SHR appeared limited to the initials and involved induction of SCR. The spatial distribution of competence to respond to SHR highlights the importance of tightly regulated movement in generating the root radial pattern.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011676480

Download citation: RISBibTeXText

PMID: 15142972

DOI: 10.1242/dev.01144


Related references

Analysis of Arabidopsis root pattern formation Tissue-specific ectopic expression of the moving putative transcription factor short-root. Developmental Biology 247(2): 486-487, 2002

The number of nodes produced by a specific genotype of Nicotiana tabacum results from interactions among root signal , leaf signal and competence of the meristem to respond to these signals. Plant Physiology (Rockville) 105(1 Suppl. ): 52, 1994

Broad competence to express er81 revealed by surgical manipultions after motoneuron identity is specified in chick. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 39 11, 2003

Region-specific variation of gene expression in the human epididymis as revealed by in situ hybridization with tissue-specific cDNAs. Molecular Reproduction and Development 34(1): 16-24, 1993

The acquisition of competence to respond to ecdysone in Drosophila is transcript specific. Mechanisms of Development 82(1-2): 131-139, 1999

Ectopic gene expression in chick mesenchyme by tissue specific electroporation. FASEB Journal 15(4): A381, 2001

Tissue-specific expression of stabilized SOLITARY-ROOT/IAA14 alters lateral root development in Arabidopsis. Plant Journal 44(3): 382-395, 2005

Tissue-specific and ectopic expression of genes introduced into transgenic mice by retroviruses. Science 234(4782): 1409-1413, 1986

Tissue-specific expression of head-to-tail cyclized miniproteins in Violaceae and structure determination of the root cyclotide Viola hederacea root cyclotide1. Plant Cell 16(8): 2204-2216, 2004

Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP. Plant Molecular Biology 50(3): 463-474, 2002

Ectopic expression of AtJMT in Nicotiana attenuata: creating a metabolic sink has tissue-specific consequences for the jasmonate metabolic network and silences downstream gene expression. Plant Physiology 157(1): 341-354, 2011

Tissue, cell type and stage-specific ectopic gene expression and RNAi induction in the Drosophila testis. Spermatogenesis 2(1): 11-22, 2012

Ectopic expression of the mycorrhiza-specific chitinase gene Mtchit 3-3 in Medicago truncatula root-organ cultures stimulates spore germination of glomalean fungi. New Phytologist 167(2): 557-570, 2005

Competence of urodele larval tissue to respond to tri iodo thyronine hormone some preliminary observations abstract amblystoma punctatum. American Zoologist 8(4): 813, 1968