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Acetylated Tat regulates human immunodeficiency virus type 1 splicing through its interaction with the splicing regulator p32



Acetylated Tat regulates human immunodeficiency virus type 1 splicing through its interaction with the splicing regulator p32



Journal of Virology 80(7): 3189-3204



The human immunodeficiency virus type 1 (HfV-1) potent transactivator Tat protein mediates pleiotropic effects on various cell functions. Posttranslational modification of Tat affects its activity during viral transcription. Tat binds to TAR and subsequently becomes acetylated on lysine residues by histone acetyltransferases. Novel protein-protein interaction domains on acetylated Tat are then established, which are necessary for both sustained transcriptional activation of the HIV-1 promoter and viral transcription-elongation. In this study, we investigated the identity of proteins that preferentially bound acetylated Tat. Using a proteomic approach, we identified a number of proteins that preferentially bound AcTat, among which p32, a cofactor of splicing factor ASF/SF-2, was identified. We found that p32 was recruited to the HIV-1 genome, suggesting a mechanism by which acetylation of Tat may inhibit HIV-1 splicing needed for the production of full-length transcripts. Using Tat from different clades, harboring a different number of acetylation sites, as well as Tat mutated at lysine residues, we demonstrated that Tat acetylation affected splicing in vivo. Finally, using confocal microscopy, we found that p32 and Tat colocalize in vivo in HIV-1-infected cells.

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Accession: 011721794

Download citation: RISBibTeXText

PMID: 16537587

DOI: 10.1128/JVI.80.7.3189-3204.2006



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