Antileukemic effect of interleukin-7-transduced bone marrow stromal cells in mice following allogeneic T-cell-depleted bone marrow transplantation
Li, A.L.; Li, C.; Feng, Y.G.; Yuan, G.H.; Wang, G.M.; Hao, J.; Gao, X.; Xie, S.S.
Transplantation Proceedings 37(5): 2297-2299
ISSN/ISBN: 0041-1345 PMID: 15964403 DOI: 10.1016/j.transproceed.2005.03.088
Impaired immune reconstitution following allogeneic bone marrow transplantation (BMT) remains a major obstacle to its clinical application. In this study, interleukin (IL)-7-transduced bone marrow stromal cells (MSC-IL7, 1 X 10(6)/mouse) were transfused into lethally irradiated C57BL/6 recipient mice. By day 40 after transplantation, the recipient mice were challenged with the lymphoma cell line EL4. MSC-IL7 cotransplantation protected recipient mice from leukemic mortality (MST > 120 days after BMT vs mean survival time (MST) 70 days in the PBS group) It enhance the PFC count and DTH responses of recipients after transplantation.