+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Association of functional influenza viral proteins and RNAs with nuclear chromatin and sub-chromatin structure



Association of functional influenza viral proteins and RNAs with nuclear chromatin and sub-chromatin structure



Microbes and Infection 8(3): 823-833



Transcription and replication of the influenza virus genome occur in the nucleus. However, the intra-nuclear localization of viral RNP complexes and the function of nuclear domains involved in viral transcription and replication, if any, are not well known. In the present study, we determined the intra-nuclear localization of viral proteins and viral RNAs and the in vitro RNA synthesis activity of viral RNP complexes associated with distinct nuclear fractions prepared from infected nuclei. A majority of viral RNA polymerases and M1 were recovered in DNase-sensitive fractions, whereas some portion of RNA polymerases and approximately 25% of NP were tightly associated with so-called nuclear matrix fractions. The amount of vRNA associated with the nuclear matrix was significantly more than that of cRNA. The in vitro viral RNA synthesis activity was detected in DNase-insensitive fractions, including the nuclear matrix. In contrast, newly synthesized viral RNAs were recovered in the DNase-sensitive fraction. These observations suggest that vRNP complexes are, at least partially, associated with densely packed chromatin, where viral transcription and replication occur, and the newly synthesized vRNP complexes to be transported into the cytoplasm are released into the nucleoplasm.

(PDF emailed within 0-6 h: $19.90)

Accession: 011786694

Download citation: RISBibTeXText

PMID: 16513387

DOI: 10.1016/j.micinf.2005.10.005


Related references

The study of chromatin and chromosome structure on preparations of interphase nucleus derivatives resulting from nuclear wall removal.III Structural heterogeneity of chromatin and argyrophilic zone of the nucleolus in stretched membrane-free nuclei and chromatin bodies from human peripheral lymphocytes. Tsitologiia 42(5): 473-485, 2000

Immunochemical study of chromatin nonhistone proteins 1. production of antibodies against rat thymus and liver chromatin analysis of tissue specificity and intra nuclear distribution of immunogenic proteins. Molecular and Cellular Biochemistry 62(2): 133-140, 1984

RNAs - physical and functional modulators of chromatin reader proteins. Biochimica et Biophysica Acta 1839(8): 737-742, 2014

Structure of eukaryotic chromosomes and chromatin part 1 on the structure of cellular and viral chromatin. Philosophical Transactions of the Royal Society of London B Biological Sciences 283(997): 274-286, 1978

Su1989 Functional Characterization of Swi/Snf Chromatin Remodelling Complex Genes Through Optical Interrogation of Nuclear Chromatin Nanoarchitecture. Gastroenterology 144(5): S-525, 2013

Nuclear protein modification and chromatin substructure. 3. Relationship between poly(adenosine diphosphate) ribosylation and different functional forms of chromatin. Biochemistry 18(6): 983-990, 1979

Chromatin assembly sites of association of newly synthesized chromatin proteins on dna. Nierlich, Donald P , W J Rutter And C Fred Fox (Ed ) Icn-Ucla Symposia on Molecular And Cellular Biology, Vol V Molecular Mechanisms in The Control Of Gene Expression Keystone, Colo , Usa, March 21-26, Xi+655p Illus Academic Press, Inc: New York, N Y , Usa; London, England ISBN 0-12-518550-2: 39-44, 1976

RNA polymerase III transcription - regulated by chromatin structure and regulator of nuclear chromatin organization. Sub-Cellular Biochemistry 61: 261-287, 2014

Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochemical and Biophysical Research Communications 52(2): 504-510, 1973

Structure of Eukaryotic Chromosomes and Chromatin || On the Structure of Cellular and Viral Chromatin. Philosophical Transactions of the Royal Society of London Series B Biological Sciences 283(997): 275-285, 1978

Dna and chromatin structure: Influence of incubation on the chromatin condensation and nuclear stability of human spermatozoa by flow cytometry. Human Reproduction 10(5): 1280-1286, 1995

Association of influenza virus ribonucleoproteins with nuclear chromatin in infected cells. Doklady Akademii Nauk SSSR 245(4): 974-976, 1979

Structure of interphase macro nuclear chromatin of a ciliate bursaria truncatella 2. loop organization of inactive chromatin clumps/. Molekulyarnaya Biologiya 18(1): 272-276, 1984

The Fun30 chromatin remodeler Fft3 controls nuclear organization and chromatin structure of insulators and subtelomeres in fission yeast. Plos Genetics 11(3): E1005101, 2015

Localization of ribosomal genes and distribution of nucleo proteins in eu chromatin and hetero chromatin fractions of rat liver chromatin. Journal of Cell Biology 67(2 PART 2): 367A, 1975