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Bioconversion of D,L-tert-leucine nitrile to D-tert-leucine by recombinant cells expressing nitrile hydratase and D-selective amidase



Bioconversion of D,L-tert-leucine nitrile to D-tert-leucine by recombinant cells expressing nitrile hydratase and D-selective amidase



Engineering in Life Sciences 4(6): 547-556



This project is aimed at studying the feasibility of producing D-tert-leucine (D-Tie) from racemic tert-leucine-nitrile (Tie-nitrile) using nitrile-transforming enzymes. For the conversion of Tle-nitrile to D-Tle, a bacterial strain, previously recovered from a deep-sea sediment, Rhodococcus erythropolis 870-AN019, was used, which expresses a nitrite hydratase (NHase)/amidase system for nitrite metabolism.

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Accession: 011806592

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DOI: 10.1002/elsc.200402153


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