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Characterization of recombinant Xenopus laevis type I iodothyronine deiodinase: substitution of a proline residue in the catalytic center by serine (Pro132Ser) restores sensitivity to 6-propyl-2-thiouracil

Kuiper, G.G.J.M.; Klootwijk, W.; Morvan Dubois, G.; Destree, O.; Darras, V.M.; Van der Geyten, S.; Demeneix, B.; Visser, T.J.

Endocrinology 147(7): 3519-3529

2006

ISSN/ISBN: 0013-7227
PMID: 16601143
DOI: 10.1210/en.2005-0711
Accession: 011850472
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In frogs such as Rana and Xenopus, metamorphosis does not occur in the absence of a functional thyroid gland. Previous studies indicated that coordinated development in frogs requires tissue and stage-dependent type II and type III iodothyronine deiodinase expression patterns to obtain requisite levels of intracellular T-3 in tissues at the appropriate stages of metamorphosis.

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Related References

Kuiper, G.G.J.M.; Klootwijk, W.; Morvan Dubois, G.; Destree, O.; Darras, V.M.; Van der Geyten, S.; Demeneix, B.; Visser, T.J. 2006: Characterization of recombinant Xenopus laevis type I lodothyronine deiodinase : Substitution of a proline residue in the catalytic center by serine (Pro132Ser) restores sensitivity to 6-propyl-2-thiouracil Endocrinology (Philadelphia) 147(7): 3519-3529
Goemann, I.M.; Gereben, B.áz.; Harney, J.W.; Zhu, B.; Maia, A.L.; Larsen, P.R. 2010: Substitution of serine for proline in the active center of type 2 iodothyronine deiodinase substantially alters its in vitro biochemical properties with dithiothreitol but not its function in intact cells Endocrinology 151(2): 821-829
Kuiper, G.G.J.M.; Klootwijk, W.; Visser, T.J. 2002: Substitution of cysteine for a conserved alanine residue in the catalytic center of type Ii iodothyronine deiodinase alters interaction with reducing cofactor Endocrinology 143(4): 1190-1198
Mayasich, S.A.; Korte, J.J.; Denny, J.S.; Hartig, P.C.; Olker, J.H.; DeGoey, P.; O'Flanagan, J.; Degitz, S.J.; Hornung, M.W. 2021: Xenopus laevis and human type 3 iodothyronine deiodinase enzyme cross-species sensitivity to inhibition by ToxCast chemicals Toxicology in Vitro: An International Journal Published in Association with Bibra 73: 105141
Kuiper, G.G.J.M.; Klootwijk, W.; Visser, T.J. 2003: Substitution of cysteine for selenocysteine in the catalytic center of type III iodothyronine deiodinase reduces catalytic efficiency and alters substrate preference Endocrinology 144(6): 2505-2513
St Germain, D.L.; Morganelli, C.M. 1989: Expression of type i iodothyronine 5'-deiodinase in Xenopus laevis oocytes Journal of Biological Chemistry 264(6): 3054-3056
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St Germain, D.L.; Schwartzman, R.A.; Croteau, W.; Kanamori, A.; Wang, Z.; Brown, D.D.; Galton, V.A. 1994: A thyroid hormone-regulated gene in Xenopus laevis encodes a type III iodothyronine 5-deiodinase Proceedings of the National Academy of Sciences of the United States of America 91(23): 11282
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