+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chiral separation in capillary electrophoresis using dual neutral cyclodextrins: theoretical models of electrophoretic mobility difference and separation selectivity

Chiral separation in capillary electrophoresis using dual neutral cyclodextrins: theoretical models of electrophoretic mobility difference and separation selectivity

Electrophoresis 26(20): 3814-3823

Simple equations and theoretical models, related to enantioselectivity (kappa) and C, have been developed for prediction of electrophoretic mobility difference (Deltamu) and separation selectivity (alpha) for enantiomers in CE using dual CDs, where alpha and kappa are defined as the ratio of mu and the ratio of binding constant (K) for enantiomers to each CD, respectively, C the CD concentration, and the average K for enantiomers and each CD. Experiments were carried out using dual CDs as beta-CD and dimethyl-beta-cyclodextrin (DM-beta-CD) and test analytes as five pairs of amphetamine drug enantiomers. A change in observed Deltamu and alpha of enantiomers in dual CDs was found to be in excellent agreement with the theoretical models. For example, in comparison with single CD1, dual CDs can enhance Deltamu and alpha up to the maximum value when enantiomers migrate with the same order in CD1 and CD2, and have the value of rho > 1.0, where rho is the enantioselectivity ratio for CD2 to CD1, while worse Deltamu and alpha are obtained for enantiomers with rho < 1.0.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 011855784

Download citation: RISBibTeXText

PMID: 16217830

DOI: 10.1002/elps.200500028

Related references

Chiral separation of salbutamol and bupivacaine by capillary electrophoresis using dual neutral cyclodextrins as selectors and its application to pharmaceutical preparations and rat blood samples assay. Journal of Chromatography. B Analytical Technologies in the Biomedical and Life Sciences 832(1): 90-96, 2006

Separation selectivity in chiral and achiral capillary electrophoresis with mixed cyclodextrins. Journal of Chromatography A 792(1-2): 297-307, 1997

Enantiomeric separation of tapentadol by capillary electrophoresis--study of chiral selectivity manipulation by various types of cyclodextrins. Journal of Pharmaceutical and Biomedical Analysis 105: 10-16, 2015

Electrophoretic separation strategy for chiral pharmaceuticals using highly-sulfated and neutral cyclodextrins based dual selector systems. Analytica Chimica Acta 525(2): 247-263, 2004

Comparison of chiral separation of basic drugs in capillary electrophoresis and liquid chromatography using neutral and negatively charged cyclodextrins. AnalyticaChimicaActa645(1-2):98, 2009

When a truck becomes a motorcycle: the impact of sample load on a chiral capillary electrophoresis separation using mixtures of neutral and sulfated cyclodextrins. Electrophoresis 20(13): 2664-2669, 1999

Chiral separation of neutral species by capillary electrophoresis. Evaluation of a theoretical model. Journal of Chromatography A 761(1-2): 249-257, 1997

Capillary zone electrophoresis for separation of drug enantiomers using cyclodextrins as chiral selectors Influence of experimental parameters on separation. Journal of Chromatography A 666(1-2): 337-350, 1994

Capillary electrophoresis of herbicides. 1. Precolumn derivatization of chiral and achiral phenoxy acid herbicides with a fluorescent tag for electrophoretic separation in the presence of cyclodextrins and micellar phases. Analytical Chemistry Washington 68(10): 1771-1777, 1996

Chiral separation of basic drugs using cyclodextrins as chiral pseudo-stationary phases in capillary electrophoresis. Journal of Chromatography 648(1): 267-274, 1993

Chiral separation of abscisic acid enantiomers by capillary zone electrophoresis using cyclodextrins and their derivatives as chiral selectors. Analytical Letters 35(13): 2185-2197, 2002

Chiral separation of hydroxyflavanones in cyclodextrin-modified capillary zone electrophoresis using sulfated cyclodextrins as chiral selectors. Journal of Chromatography. a 1188(2): 301-307, 2008

Separation of chiral isomers of p-nitrophenyl-2-amino-3-hydroxypropanone by capillary zone electrophoresis using cyclodextrins as chiral selector. Journal of Chromatography A 667(1-2): 367-370, 1994

Chiral separation of lobeline analogs using high performance capillary electrophoresis and derivatized cyclodextrins as chiral additives. Electrophoresis 20(1): 212-218, 1999

Use of cyclodextrins as chiral selector for the chiral separation of anticholinergic drugs such as anisodamine and glycopyrronium in capillary zone electrophoresis. HRC Journal of High Resolution Chromatography 19(12): 697-699, 1996