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Cloning and analysis of the full-length cDNA sequence and 5 '-flanking region of silver carp soluble glutathione S-transferase gene



Cloning and analysis of the full-length cDNA sequence and 5 '-flanking region of silver carp soluble glutathione S-transferase gene



Zhongguo Shengwu Huaxue yu Fenzi Shengwu Xuebao 22(6): 470-476



Soluble glutathione S-transferase (sGST) of freshwater fish is extremely important to microcystin purification from fish body, and is therefore also named microcystin-detoxifizyme. PCR using two degenerated primers, yielded a cDNA fragment of 591 bp from the liver of a phytoplanktivorous freshwater fish, silver carp (Hypophthalmichthys molitrix), which consumed substantial amounts of toxic blue-green algae in the food. The full-length cDNA fragment was obtained by 5' and 3' RACE.

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