Cloning of celC, third cellulase gene, from Pectobacterium carotovorum subsp carotovorum LY34 and its comparison to those of Pectobacterium sp

Lim; Jin, W.; Ryu, S.-Kee; Park, S.-Ryeol; Kim, M.-Keun; An, C.-Long; Hong, S.-Young; Shin, E.-Chule; Lee, J.-Yeoul; Lim, Y.-Pyo; Yun, H.-Dae

Journal of Microbiology and Biotechnology 15(2): 302-309


ISSN/ISBN: 1017-7825
Accession: 011868955

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Phytopathogenic Pectobacterium carotovorum subsp. carotovorum (Pcc) LY34 secretes multiple isozymes of the plant cell wall degrading enzyme endoglucanases. We have cloned a third cel gene encoding CMCase from Pcc LY34. The structural organization of the celC gene (AY 188753) consisted of an open reading frame (ORF) of 1, 116 bp encodinor 371 amino acid residues with a signal peptide of 22 amino acids within the NH2-terminal region of pre-CeIC. The predicted amino acid sequence of CeIC was similar to that of Pectobacterium chrysanthemi Ce18Y (AF282321). The CeIC has the conserved region of the glycoside hydrolase family 8. The apparent molecular mass of CeIC was calculated to be 39 kDa by CMC-SDS-PAGE. The cellulase-minus mutant of Pcc LY34 was as virulent as the wild-type in pathogenicity tests on tubers of potato. The results suggest that the CeIC of Pcc LY34 is a minor factor for the pathogenesis of soft-rot.