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Cloning, characterisation, and expression of three oestrogen receptors (ERalpha, ERbeta1 and ERbeta2) in the European sea bass, Dicentrarchus labrax



Cloning, characterisation, and expression of three oestrogen receptors (ERalpha, ERbeta1 and ERbeta2) in the European sea bass, Dicentrarchus labrax



Molecular and Cellular Endocrinology 223(1-2): 63-75



Three oestrogen receptor [ER] subtypes have been described in teleost fish, namely ER alpha , and two ER beta subtypes, called ER beta 1 and ER beta 2 (or ER beta and ER gamma in Atlantic croaker). Their expression during embryonic development and gonadal growth has evoked interest in their potential role in sexual differentiation and gonadal development in fish. We cloned three oestrogen receptors from adult liver (sb-ER alpha cDNA) and ovary (partial sb-ER beta 1 and sb-ER beta 2 cDNAs) of the European sea bass, and according to their phylogenetic relatedness to other ERs in teleosts, named them sea bass [sb-] ER alpha , ER beta 1 and ER beta 2. Deduced amino acid numbers for sb-ER alpha , sb-ER beta 1 and sb-ER beta 2 were 639, 517 and 608, respectively, representing in the case of sb-ER beta 1 and sb-ER beta 2 about 90% of the open reading frame. Highest amino acid identities were found for sb-ER alpha with eelpout ER alpha (88.7%), for sb-ER beta 1 with Atlantic croaker ER gamma (85.8%), and for sb-ER beta 2 with Atlantic croaker ER beta (90.1%). Southern analysis confirmed that all three sea bass oestrogen receptors (sb-ERs) are the products of three distinct genes. In adult sea bass, ER alpha was predominantly expressed in liver and pituitary, while sb-ER beta 1 and sb-ER beta 2 were more ubiquitously expressed, with highest expression levels in pituitary. In a mixed-sex population of juvenile sea bass, sb-ER alpha expression was significantly elevated in gonads at 200 days posthatch (dph), while for sb-ER beta 1 and sb-ER beta 2 highest expression levels were observed in gonads at 250 dph. For sb-ER beta 2, expression was also significantly higher in the brain at 250 dph. The cloning of these three ER subtypes in the European sea bass together with the results obtained on expression levels in adult and juvenile animals has given us the foundation to investigate their possible role in sexual differentiation and development in this species in future studies.

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Accession: 011869145

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PMID: 15279912

DOI: 10.1016/j.mce.2004.05.009


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