+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564: preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes



Control of the nitrile-hydrolyzing enzyme activity in Rhodococcus rhodochrous IFO 15564: preferential action of nitrile hydratase and amidase depending on the reaction condition factors and its application to the one-pot preparation of amides from aldehydes



Journal of Molecular Catalysis B Enzymatic 29(1-6): 249-258



The reaction conditions towards the preferential action of either nitrile hydratase or amidase in the harvested whole cells of Rhodococcus rhodochrous IFO 15564 were elaborated. The amidase showed higher heat tolerance than the nitrile hydratase and, at 45degreeC the amidase worked exclusively. DMSO assisted the preferential action of nitrile hydratase, however, at more than 30% (v/v) addition of DMF, the nitrile hydratase activity was completely lost and only amidase worked. A one-pot chemo-enzymatic conversion of aldehydes to amides ((1) aq. NH3, I2, DMSO; (2) Na2S2O3; (3) harvested cells of R. rhodochrous) was established. Under these reaction conditions, most of the amidase was lost, and the incubation of the firstly formed intermediates, nitriles in aq. NH3 was responsible for the selective inhibition of amidase. The freezing of harvested cells in an exhaustively deionized environment provided a long-term preservable "ready to use" for the organic chemist.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011901581

Download citation: RISBibTeXText

DOI: 10.1016/j.molcatb.2003.12.014


Related references

Action of Nitrile Hydratase from Rhodococcus rhodochrous Ifo 15564 on Derivatives of 2,5-Anhydro- d -allononitrile. Bioscience, Biotechnology and Biochemistry 60(9): 1540-1542, 1996

Action of nitrile hydratase from Rhodococcus rhodochrous IFO 15564 on derivatives of 2,5-anhydro-D-allononitrile. Bioscience Biotechnology & Biochemistry 60(9): 1540-1542, 1996

Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771. Journal of Bioscience and Bioengineering 97(4): 250-259, 2004

Nitrile hydratase and amidase from Rhodococcus rhodochrous hydrolyze acrylic fibers and granular polyacrylonitriles. Applied and Environmental Microbiology 66(4): 1634-1638, 2000

Amidase coupled with low-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1: Sequencing and expression of the gene and purification and characterization of the gene product. European Journal of Biochemistry 217(1): 327-336, 1993

Enzyme-substrate binding landscapes in the process of nitrile biodegradation mediated by nitrile hydratase and amidase. Applied Biochemistry and Biotechnology 170(7): 1614-1623, 2013

The respiratory activity of Rhodococcus rhodochrous M8 cells producing nitrile-hydrolyzing enzymes. Prikladnaia Biokhimiia i Mikrobiologiia 37(3): 326-331, 2001

Diversity of nitrile hydratase and amidase enzyme genes in Rhodococcus erythropolis recovered from geographically distinct habitats. Applied and Environmental Microbiology 69(10): 5754-5766, 2003

Characterization of the gene cluster of high-molecular-mass nitrile hydratase (H-NHase) induced by its reaction product in Rhodococcus rhodochrous J1. Proceedings of the National Academy of Sciences of the United States of America 93(9): 4267-4272, 1996

Nitrile, amide and temperature effects on amidase-kinetics during acrylonitrile bioconversion by nitrile-hydratase/amidase in situ cascade system. Bioresource Technology 142: 320-328, 2013

Overexpression of high-molecular-mass nitrile hydratase from Rhodococcus rhodochrous J1 in recombinant Rhodococcus cells. Applied Microbiology & Biotechnology. 49(5): 568-572,, 1998

Expression control of nitrile hydratase and amidase genes in Rhodococcus erythropolis and substrate specificities of the enzymes. Antonie van Leeuwenhoek 105(6): 1179-1190, 2014

Strain of Rhodococcus rhodochrous as a producer of nitrile hydratase. Official Gazette of the United States Patent & Trademark Office Patents 1215(4): 4076, 1998

A novel gene cluster including the Rhodococcus rhodochrous J1 nhlBA genes encoding a low molecular mass nitrile hydratase (L-NHase) induced by its reaction product. Journal of Biological Chemistry 271(26): 15796-15802, 1996

Effects of nitriles and amides on the growth and the nitrile hydratase activity of the Rhodococcus sp. strain gt1. Prikladnaia Biokhimiia i Mikrobiologiia 39(1): 63-68, 2003