+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Crystallization and preliminary crystallographic analysis of two Streptococcus agalactiae proteins: the family II inorganic pyrophosphatase and the serine/threonine phosphatase



Crystallization and preliminary crystallographic analysis of two Streptococcus agalactiae proteins: the family II inorganic pyrophosphatase and the serine/threonine phosphatase



Acta Crystallographica. Section F Structural Biology and Crystallization Communications 62(Pt 9): 891-894



Streptococcus agalactiae, which infects human neonates and causes sepsis and meningitis, has recently been shown to possess a eukaryotic-like serine/threonine protein phosphorylation signalling cascade. Through their target proteins, the S. agalactiae Ser/Thr kinase and Ser/Thr phosphatase together control the growth as well as the morphology and virulence of this organism. One of the targets is the S. agalactiae family II inorganic pyrophosphatase. The inorganic pyrophosphatase and the serine/threonine phosphatase have therefore been purified and crystallized and diffraction data have been collected from their crystals. The data were processed using XDS. The inorganic pyrosphosphatase crystals diffracted to 2.80 A and the Ser/Thr phosphatase crystals to 2.65 A. Initial structure-solution experiments indicate that structure solution will be successful in both cases. Solving the structure of the proteins involved in this cascade is the first step towards understanding this phenomenon in atomic detail.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011913176

Download citation: RISBibTeXText

PMID: 16946472

DOI: 10.1107/s174430910602954x


Related references

A eukaryotic type serine/threonine kinase and phosphatase in Streptococcus agalactiae reversibly phosphorylate an inorganic pyrophosphatase and affect growth, cell segregation, and virulence. Journal of Biological Chemistry 278(16): 14429-14441, 2003

Expression, purification, crystallization and preliminary crystallographic analysis of laminin-binding protein (Lmb) from Streptococcus agalactiae. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 5): 492-494, 2009

Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 A resolution. Acta Crystallographica. Section D Biological Crystallography 63(Pt 6): 738-743, 2007

Structure of the Streptococcus agalactiae family II inorganic pyrophosphatase at 2.80 angstrom resolution. Acta Crystallographica Section D Biological Crystallography 63(Part 6): 738-743, 2007

Crystallization and preliminary crystallographic analysis of bifunctional gamma-glutamylcysteine synthetase-glutatione synthetase from Streptococcus agalactiae. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 7): 678-680, 2009

Structure of Streptococcus agalactiae serine/threonine phosphatase. The subdomain conformation is coupled to the binding of a third metal ion. Febs Journal 274(12): 3128-3137, 2007

Expression, purification, crystallization and preliminary X-ray diffraction studies of the human keratin 4-binding domain of serine-rich repeat protein 1 from Streptococcus agalactiae. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 12): 1582-1585, 2011

Purification, crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from Thermus thermophilus. Journal of Molecular Biology 232(1): 312-313, 1993

Crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. Acta Crystallographica. Section D Biological Crystallography 60(Pt 3): 577-579, 2004

Crystallization and preliminary X-ray crystallographic analysis of biodegradative threonine deaminase (TdcB) from Salmonella typhimurium. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 62(Pt 3): 275-278, 2006

Crystallization and preliminary crystallographic analysis of human serine dehydratase. Acta Crystallographica. Section D Biological Crystallography 59(Pt 12): 2297-2299, 2003

Crystallization and preliminary crystallographic analysis of D-serine dehydratase from chicken kidney. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 1): 147-149, 2011

Crystallization and preliminary crystallographic analysis of dextranase from Streptococcus mutans. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 12): 1542-1544, 2011

Crystallization and preliminary crystallographic analysis of recombinant hyaluronate lyase from Streptococcus suis. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 69(Pt 6): 673-675, 2013