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Determination of beta-exotoxin in Iranian isolates of Bacillus thuringiensis


Determination of beta-exotoxin in Iranian isolates of Bacillus thuringiensis



Journal of Entomological Society of Iran 23(1): 55-68



Twelve Iranian isolates of B. thuringiensis were evaluated for beta-exotoxin using combination of bioassay and HPLC methods. Bioassay results on 4 days old larvae of Helicoverpa armigera showed the existence of a mortality factor, which can be beta-exotoxin, in some autoclaved supernatant of isolates (with no spores and crystals). To verify these results, the beta-exotoxin was measured in each isolate using HPLC method. The results indicated that the concentrated supernatant of isolates Krm23, Kn4, 3R and 4t contained beta-exotoxin. Isolates Krm23 and 3R had the lowest (7.37 mug/ml) and highest (294.5 mug/ml) amounts of beta-exotoxin, respectively. Comparison of bioassay results with beta-exotoxin analyzed by HPLC showed that measured quantities of beta-exotoxin were confirmed by the mortality percentage of Helicoverpa armigera.

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