+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Development of a novel electrokinetically driven microfluidic immunoassay for the detection of Helicobacter pylori



Development of a novel electrokinetically driven microfluidic immunoassay for the detection of Helicobacter pylori



Analytica Chimica Acta 543(1-2): 109-116



A novel microchip-based electrokinetically driven immunoassay was developed in this study. The microchip was made of poly(dimethylsitoxane) (PDMS)/glass using photolithography and replica molding. The immunoreaction was between the anti-Helicobacter pylori antibody and the bacterial protein antigen immobilized on the wall of the microchannel. The conditions for antigen immobilization were optimized by adjusting the plasma treatment time.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011944771

Download citation: RISBibTeXText

DOI: 10.1016/j.aca.2005.04.032


Related references

Development of a novel microfluidic immunoassay for the detection of Helicobacter pylori infection. Analyst 129(9): 823-828, 2004

Development of a lateral flow immunoassay strip for rapid detection of CagA antigen of Helicobacter pylori. Journal of Immunoassay and Immunochemistry 36(3): 324-333, 2015

Electrokinetically driven microfluidic chips with surface-modified chambers for heterogeneous immunoassays. Analytical Chemistry 73(14): 3400-3409, 2001

A programmable and configurable multi-port System-on-Chip for stimulating electrokinetically-driven microfluidic devices. Conference Proceedings 2011: 8361-8364, 2011

Enhancement of electrokinetically driven microfluidic T-mixer using frequency modulated electric field and channel geometry effects. Electrophoresis 30(18): 3144-3152, 2009

Electrokinetically driven fluidic transport in integrated three-dimensional microfluidic devices incorporating gold-coated nanocapillary array membranes. Lab on a Chip 8(10): 1625-1631, 2008

Using enzyme immunoassay to detect Helicobacter pylori stool antigen for investigating the prevalence of Helicobacter pylori infection in children and adolescents in Changhua. Acta Paediatrica Taiwanica 43(3): 133-139, 2002

Detection of Helicobacter pylori antigen in faeces by enzyme immunoassay. Pathology 33(4): 496-497, 2001

Detection of Helicobacter pylori antigen in faeces by enzyme immunoassay. Pathology 33(4): 496-497, 2001

Detection of Helicobacter pylori in faeces by culture, PCR and enzyme immunoassay. Journal of Medical Microbiology 50(12): 1021-1029, 2001

Detection of Helicobacter pylori antigen in stool by enzyme immunoassay. Yonsei Medical Journal 43(1): 7-13, 2002

Usefulness of a novel enzyme immunoassay for the detection of Helicobacter pylori in feces. Scandinavian Journal of Gastroenterology 35(1): 49-53, 2000

Integrated electrokinetically driven microfluidic devices with pH-mediated solid-phase extraction coupled to microchip electrophoresis for preterm birth biomarkers. Electrophoresis 38(13-14): 1743-1754, 2017

Quantitative Detection of Digoxin in Plasma Using Small-Molecule Immunoassay in a Recyclable Gravity-Driven Microfluidic Chip. Advanced Science 6(6): 1802051, 2019

Detection of cytoplasmic proteins from Helicobacter pylori in Colony Lift Immunoassay. Journal of Microbiological Methods 119: 145-146, 2015