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Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activity

Monnet, C.; Aymes, F.; Corrieu, G.

Applied and Environmental Microbiology 66(12): 5518-5520

2000

ISSN/ISBN: 0099-2240
PMID: 11097941
DOI: 10.1128/aem.66.12.5518-5520.2000
Accession: 011949319
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Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha-acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin.

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Related References

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