+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Drosophila Paf1 modulates chromatin structure at actively transcribed genes



Drosophila Paf1 modulates chromatin structure at actively transcribed genes



Molecular and Cellular Biology 26(1): 250-260



The Paf1 complex in yeast has been reported to influence a multitude of steps in gene expression through interactions with RNA polymerase II (Pol II) and chromatin-modifying complexes; however, it is unclear which of these many activities are primary functions of Paf1 and are conserved in metazoans. We have identified and characterized the Drosophila homologs of three subunits of the yeast Paf1 complex and found striking differences between the yeast and Drosophila Paf1 complexes. We demonstrate that although Drosophila Paf1, Rtf1, and Cdc73 colocalize broadly with actively transcribing, phosphorylated Pol II, and all are recruited to activated heat shock genes with similar kinetics; Rtf1 does not appear to be a stable part of the Drosophila Paf1 complex. RNA interference (RNAi)-mediated depletion of Paf1 or Rtf1 leads to defects in induction of Hsp70 RNA, but tandem RNAi-chromatin immunoprecipitation assays show that loss of neither Paf1 nor Rtf1 alters the density or distribution of phosphorylated Pol II on the active Hsp70 gene. However, depletion of Paf1 reduces trimethylation of histone H3 at lysine 4 in the Hsp70 promoter region and significantly decreases the recruitment of chromatin-associated factors Spt6 and FACT, suggesting that Paf1 may manifest its effects on transcription through modulating chromatin structure.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 011978294

Download citation: RISBibTeXText

PMID: 16354696

DOI: 10.1128/mcb.26.1.250-260.2006


Related references

The endoglycosidase heparanase enters the nucleus of T lymphocytes and modulates H3 methylation at actively transcribed genes via the interplay with key chromatin modifying enzymes. Transcription 3(3): 130-145, 2012

Inhibition of topoisomerase i increases the release of actively transcribed genes from chromatin. Journal of Cell Biology 107(6 Part 3): 313, 1988

Chromatin structure of ribosomal genes of Drosophila melanogaster. Random location of nucleosomes in DNA and characteristics of organization of non-transcribed spacer. Molekuliarnaia Biologiia 18(4): 1141-1150, 1984

The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin. Cell Reports 20(10): 2313-2327, 2017

Actively transcribed GAL genes can be physically linked to the nuclear pore by the SAGA chromatin modifying complex. Journal of Biological Chemistry 282(5): 3042-3049, 2007

MSL complex associates with clusters of actively transcribed genes along the Drosophila male X chromosome. Cold Spring Harbor Symposia on Quantitative Biology 71: 385-394, 2006

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5' end of the actively transcribed ovalbumin gene. Embo Journal 1(2): 223-230, 1982

Disruption of the typical chromatin structure in a 2500 base-pair region at the 5 end of the actively transcribed ovalbumin gene. The Embo Journal 1(2): 223-230, 1982

The ATP-dependent chromatin remodeler Chd1 is recruited by transcription elongation factors and maintains H3K4me3/H3K36me3 domains at actively transcribed and spliced genes. Nucleic Acids Research 45(12): 7180-7190, 2017

Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. Genes & Development 22(9): 1190-1204, 2008

Ultrastructure of actively transcribed chicken ov albumin genes chromatin analysis of ov albumin gene injected xenopus oocyte nuclei. European Journal of Cell Biology 22(1): 29, 1980

Drosophila PAF1 Modulates PIWI/piRNA Silencing Capacity. Current Biology 27(17): 2718-2726.E4, 2017

The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast. Embo Reports 16(12): 1673-1687, 2015

Unusual chromatin structure of transcribed and nontranscribed segments of mouse ribosomal rna genes. Journal of Cellular Biochemistry Suppl. (6): 325, 1982

Use of the modified method of chromatin immunoprecipitation for the isolation of actively transcribed loci. Molekuliarnaia Biologiia 37(1): 156-163, 2003