Early genetic effects on workers occupationally exposed to formaldehyde

Yu, L-qun.; Jiang, S-fang.; Leng, S-guang.; He, F-sheng.; Zheng, Y-xin.

Zhonghua Yu Fang Yi Xue Za Zhi 39(6): 392-395

2005


ISSN/ISBN: 0253-9624
PMID: 16329798
Accession: 011986001

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Abstract
Objective To investigate the DNA and chromosome damage in peripheral blood lymphocyte of workers occupationally exposed to formaldehyde ( FA). Methods All 151 workers occupationally exposed to FA from two plywood factories and 112 workers without occupational FA exposure working in a machine manufactory were recruited into this study. Comet assay and cytokinesis-block micronucleus technique was used to evaluate the DNA and chromosomal damage of peripheral blood lymphocyte. The air FA samples were collected with SKC 224-PCXR8 air samplers. Gas chromatography was used to analyze the FA level. Personal information including occupational history, age, sex, smoking and drinking status was collected by the questionnaire. Results The time weighted average concentration (TWA) of FA in the working environment of FA-exposed workers (range 0.10-7.88 mg/m(3)) was higher than those in controls (<0.01 mg/m(3)). The olive tail moment (Olive TM) in low FA-exposed workers [3.03(2.49-3.67)] was lower than that in high FA-exposed workers [3.95(3.53-4.43)], but higher than that in controls [0.93(0.78-1.10)], the differences were statistical significant (P<0.05). Comet trail length in FA-exposed workers were significantly higher than that in controls (6.78(6.05-7.60)], but no significant differences ware found between the high FA-exposed workers (12.59(11.80-13.43)] and the low FA-exposed workers [11.25(10.12-12.50)]. The frequency of micronuclei per 100 binucleated cells in low FA-exposed workers (0.41 +/- 0.25) was lower than that in high FA-exposed workers (0.65 +/- 0.36), but higher than that in controls (0.27 +/- 0.13), the differences were statistical significant (P<0.05). The increased tendencies with the exposure levels were found in those three indices. In stratification analysis, the same results were found. Conclusion In the current FA exposure levels, the DNA and chromosomal damage in peripheral blood lymphocyte might be induced by FA exposure, and be increased with the levels of exposure.