+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effect of inhaled N-acetylcysteine on hydrogen peroxide exhalation in healthy subjects



Effect of inhaled N-acetylcysteine on hydrogen peroxide exhalation in healthy subjects



Pulmonary Pharmacology and Therapeutics 17(3): 155-162



N-acetylcysteine (NAC) has antioxidant properties and its oral administration decreased H(2)O(2) exhalation in patients with chronic obstructive pulmonary disease. In this study we tested whether inhaled NAC could suppress H(2)O(2) levels in exhaled breath condensate (EBC) of eight healthy subjects that have never smoked (never-smokers). Original NAC solution (ACC vial, 300 mg NAC in 3 ml solvent), NAC-placebo (vehicle), sterile 0.9% NaCl or distilled water were nebulized via the pneumatic De Vilbiss nebulizer once daily every 7 days and H(2)O(2) and thiols exhalation was measured just before, 30 min and 3 h after the end of drug administration. Additional in vitro experiments were performed to evaluate NAC stability during nebulization, reactivity with H(2)O(2) and possible H(2)O(2) generation in aqueous NAC solutions. NAC almost completely abolished H(2)O(2) exhalation 30 min after inhalation (0.02+/-0.04 vs. 0.21+/-0.09 microM, p<0.001). However, 3 h later the H(2)O(2) levels raised 1.8-fold from baseline (p<0.01). Other inhaled solutions did not affect H(2)O(2) levels. Mean thiol concentration in EBC rose (p<0.05) after treatment with NAC and reached 1.03+/-0.48 microM at 3 h. Although, 25 and 50 mM NAC completely inhibited H(2)O(2)-peroxidase-luminol-dependent chemiluminescence, detectable amounts of H(2)O(2) were generated in NAC solutions. It was accompanied by moderate loss of -SH groups. Catalase and ascorbic acid prevented H(2)O(2) formation in NAC solutions. In conclusion inhaled NAC revealed biphasic effect on H(2)O(2) exhalation in healthy subjects, which depends on direct H(2)O(2) scavenging and H(2)O(2) generation related to drug oxidation. The net result of these processes may determine anti- or pro-oxidant action of inhaled NAC.

(PDF emailed within 0-6 h: $19.90)

Accession: 011997905

Download citation: RISBibTeXText

PMID: 15123225

DOI: 10.1016/j.pupt.2004.01.007


Related references

Long-term administration of N-acetylcysteine decreases hydrogen peroxide exhalation in subjects with chronic obstructive pulmonary disease. Respiratory Medicine 95(6): 448-456, 2001

Hydrogen peroxide exhalation in healthy never smoking subjects. European Respiratory Journal 12(SUPPL 28): 286S, Sept, 1998

Increased exhalation of hydrogen peroxide in healthy subjects following cigarette consumption. Sao Paulo Medical Journal 118(4): 93-98, 2000

Exhalation of hydrogen peroxide in COPD patients with continuous use of prednisone and/or N-acetylcysteine. European Respiratory Journal Supplement 10(25): 415S, Sept, 1997

Assessment of reproducibility of exhaled hydrogen peroxide concentration and the effect of breathing pattern in healthy subjects. Journal of Aerosol Medicine and Pulmonary Drug Delivery 24(6): 271-275, 2012

The effect of hydrogen peroxide-induced oxidative stress on leukocytes depends on age and physical training in healthy human subjects carrying the same genotypes of antioxidant enzymes' gene polymorphisms. American Journal of Human Biology 22(6): 807-812, 2011

Apocynin decreases hydrogen peroxide and nitrate concentrations in exhaled breath in healthy subjects. Pulmonary Pharmacology and Therapeutics 23(1): 48-54, 2010

Elevated exhalation of hydrogen peroxide in patients with systemic sclerosis. European Journal of Clinical Investigation 33(3): 274-279, March, 2003

Exhaled hydrogen peroxide correlates with the release of reactive oxygen species by blood phagocytes in healthy subjects. Respiratory Medicine 97(6): 718-725, 2003

Elevated exhalation of hydrogen peroxide and circulating IL-18 in patients with pulmonary tuberculosis. Respiratory Medicine 101(3): 574-580, 2006

Effect of intravenous N-acetylcysteine infusion on haemostatic parameters in healthy subjects. Gut 54(4): 515-521, 2005

DNA repair kinetic of hydrogen peroxide and UVA/B induced lesions in peripheral blood leucocytes from xeroderma pigmentosum patients and healthy subjects. Journal of Environmental Pathology, Toxicology and Oncology 33(4): 279-293, 2015

Hydrogen peroxide release and acid-base status in exhaled breath condensate at rest and after maximal exercise in young, healthy subjects. European Journal of Medical Research 14 Suppl 4: 134-139, 2010

The in vitro effect of N-acetylcysteine on prothrombin time in plasma samples from healthy subjects. Academic Emergency Medicine 18(4): 351-354, 2011

Elevated exhalation of hydrogen peroxide in patients with non-small cell lung cancer is not affected by chemotherapy. Redox Report 22(6): 308-314, 2016