+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of adrenergic agents on rat peritoneal macrophages activated in vitro by acetylated low-density lipoprotein



Effects of adrenergic agents on rat peritoneal macrophages activated in vitro by acetylated low-density lipoprotein



Pathology Research and Practice 201(5): 391-398



Macrophages interact with modified lipoproteins and alter their functional status. In this study, the effects of the adrenergic agents adrenaline, isoproterenol, and dobutamine on macrophages activated with acetylated low-density lipoprotein were tested. The aim of this investigation was to determine whether adrenergic agents influence biologically significant functions of these cells in an in vitro model of macrophage-lipoprotein acute interaction. Rat peritoneal macrophages were incubated with acetylated low-density lipoprotein for 16 h, with or without adrenergic agents. Hydrogen peroxide and nitric oxide production and acid phosphatase activities in the supernatant and cell lysate were assayed. Adrenaline and isoproterenol inhibited the production of hydrogen peroxide, stimulated the production of nitric oxide, and increased the extracellular activity of acid phosphatase in the lipoprotein-activated cells. Dobutamine increased the extracellular, but decreased the intracellular acid phosphatase activity. Adrenaline and dobutamine also produced changes in the cell size and nuclear morphology of the macrophages. Macrophages are activated in vitro by acetylated low-density lipoprotein, and their functions and morphology are modified under the influence of adrenergic agents. Certain effects could be attributed to changes in cyclic AMP levels.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012009914

Download citation: RISBibTeXText

PMID: 16047949

DOI: 10.1016/j.prp.2005.04.002


Related references

Effects of diltiazem on acetylated low density lipoprotein metabolism in cultured mouse peritoneal macrophages. Japanese Circulation Journal 53(8): 900, 1989

Effects of lysosomal protease inhibitors on the degradation of acetylated low density lipoprotein in cultured rat peritoneal macrophages. Journal of Atherosclerosis and Thrombosis 1(1): 41-44, 1994

The effects of phosphoinositide/calcium- or cyclic AMP-mediated signal transduction pathway inhibitors on the activation of rat peritoneal macrophages by acetylated low-density lipoprotein. In Vivo 18(5): 653-659, 2004

Acetylated low-density lipoprotein is endocytosed through coated pits by rat peritoneal macrophages. Virchows Archiv. B Cell Pathology Including Molecular Pathology 52(1): 1-13, 1986

Probucol does not alter acetylated low density lipoprotein uptake by murine peritoneal macrophages. Atherosclerosis 80(3): 191-197, 1990

Essential fatty acid deficiency lowers the activity of the acetylated low density lipoprotein receptor of rat peritoneal macrophages. Biochemistry & Cell Biology 70(3-4): 224-227, 1992

Involvement of calcium and arachidonate metabolism in acetylated-low-density-lipoprotein-stimulated tumor-necrosis-factor-a production by rat peritoneal macrophages. European Journal of Biochemistry 253(1): 5-53, 1998

Acetylated low density lipoprotein inhibits the incorporation of arachidonic acid in phospholipids with a concomitant increase of cholesterol arachidonate in rat peritoneal macrophages. Biochimica et Biophysica Acta 1259(3): 211-219, 1995

Involvement of calcium and arachidonate metabolism in acetylated-low-density-lipoprotein-stimulated tumor-necrosis-factor-alpha production by rat peritoneal macrophages. European Journal of Biochemistry 253(1): 345-353, 1998

Regulation of high density lipoprotein receptor activity by monocyte derived macrophages with acetylated low density lipoprotein loading. Neurology 36(4 Suppl. 1): 258, 1986

Receptor activities for low-density lipoprotein and acetylated low-density lipoprotein in a mouse macrophage cell line (IC21) and in human monocyte-derived macrophages. Journal of Experimental Medicine 154(6): 1852-1867, 1981

Topography and dynamics of receptors for acetylated and malondialdehyde-modified low-density lipoprotein in the plasma membrane of mouse peritoneal macrophages as visualized by colloidal gold in conjunction with surface replicas. Journal of Histochemistry and Cytochemistry 32(10): 1017-1027, 1984

Exposure of rat peritoneal macrophages to acetylated low density lipoprotein results in release of plasma membrane cholesterol an efficient substrate for esterification by acyl coa cholesterol acyltransferase. Journal of Biological Chemistry 267(3): 1603-1608, 1992

Toxicity of oxidised low density lipoprotein towards mouse peritoneal macrophages in vitro. Atherosclerosis 98(1): 17-24, 1993