+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effects of structural variation in xyloglucan polymers on interactions with bacterial cellulose



Effects of structural variation in xyloglucan polymers on interactions with bacterial cellulose



American Journal of Botany 93(10): 1402-1414



A cellulose/xyloglucan framework is considered to form the basis for the mechanical properties of primary plant cell walls and hence to have a major influence on the biomechanical properties of growing, fleshy plant tissues. In this study, structural variants of xyloglucan have been investigated as components of composites with bacterial cellulose as a simplified model for the cellulose/xyloglucan framework of primary plant cell walls. Evidence for molecular binding to cellulose with perturbation of cellulose crystallinity was found for all xyloglucan types. High molecular mass samples gave homogeneous centimeter-scale composites with extensive cross-linking of cellulose with xyloglucan. Lower molecular mass xyloglucans gave heterogeneous composites having a range of microscopic structures with little, if any, cross-linking. Xyloglucans with reduced levels of galactose substitution had evidence of self-association, competitive with cellulose binding. At comparable molecular mass, fucose substitution resulted in a modest promotion of microscopic features characteristic of primary cell walls. Taken together, the data are evidence that galactose substitution of the xyloglucan core structure is a major determinant of cellulose composite formation and properties, with additional fucose substitution acting as a secondary modulator. These conclusions are consistent with reported structural and mechanical properties of Arabidopsis mutants lacking specific fucose and/or galactose residues.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012022887

Download citation: RISBibTeXText

PMID: 21642087

DOI: 10.3732/ajb.93.10.1402


Related references

Effects of Structural Variation in Xyloglucan Polymers on Interactions with Bacterial Cellulose. American Journal of Botany 93(10): 1402-1414, 2006

Pea Xyloglucan and Cellulose: Vi. Xyloglucan-Cellulose Interactions in Vitro and in Vivo. Plant Physiology 83(2): 384-389, 1987

Pea Xyloglucan and Cellulose: V. Xyloglucan-Cellulose Interactions in vitro and In vivo. Plant Physiology 83(2): 384-389, 1987

Pea xyloglucan and cellulose v. xyloglucan cellulose interactions in vitro and in vivo. Plant Physiology 83(2): 384-389, 1987

The adsorption of xyloglucan on cellulose: effects of explicit water and side chain variation. Carbohydrate Research 346(16): 2595-2602, 2011

Molecular dynamics simulation study of xyloglucan adsorption on cellulose surfaces: effects of surface hydrophobicity and side-chain variation. Cellulose 21(2): 1025-1039, 2014

Enthalpic studies of xyloglucan-cellulose interactions. Biomacromolecules 11(6): 1417-1428, 2010

Bacterial cellulose nanocrystals: impact of the sulfate content on the interaction with xyloglucan. Cellulose 22(3): 1773-1787, 2015

Top-down grafting of xyloglucan to gold monitored by QCM-D and AFM: enzymatic activity and interactions with cellulose. Biomacromolecules 9(3): 942-948, 2008

Roles of xyloglucan and pectin on the mechanical properties of bacterial cellulose composite films. Cellulose 21(1): 275-289, 2014

Property evaluations of dry-cast reconstituted bacterial cellulose/tamarind xyloglucan biocomposites. Carbohydrate Polymers 93(1): 144-153, 2013

Methods for structural characterization of the products of cellulose- and xyloglucan-hydrolyzing enzymes. Methods in Enzymology 510: 121-139, 2012

Xyloglucan and xyloglucan endo-transglycosylases (XET): Tools for ex vivo cellulose surface modification. Biocatalysis and Biotransformation 24(1-2): 107-120, 2006

Localization of xyloglucan in the macromolecular complex composed of xyloglucan and cellulose in pea stems. Plant & Cell Physiology 35(3): 439-444, 1994

Xyloglucan-cellulose interaction depends on the sidechains and molecular weight of xyloglucan. Plant Physiology and Biochemistry 42(5): 389-394, 2004