+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Electron microscopy renders the diagnostic capabilities of cytopathology more precise: an approach to everyday practice



Electron microscopy renders the diagnostic capabilities of cytopathology more precise: an approach to everyday practice



Ultrastructural Pathology 29(6): 475-482



Cytology is a powerful diagnostic tool but to make definitive diagnoses, the use of ancillary techniques is imperative. By combining immunohistochemistry (IHC) and electron microscopy (EM), cytologic diagnoses can be as precise as those of surgical pathology. In the authors' daily practice of cytopathology they use all ancillary techniques available to them: histochemistry, IHC, EM, flow cytometry, and molecular pathology. IHC is frequently used as an ancillary technique in their daily practice but EM is many times their technique of choice. By the use of EM the authors can make specific final diagnoses, make the diagnosis more definitive, narrow the differential diagnosis, or determine the origin of a neoplasm with unknown primary site. Specimens obtained by fine-needle aspiration as well as all body fluids are suitable for EM. The limiting factor is to obtain the appropriate material with the diagnostic cells for ultrastructural examination. The common diagnostic dilemmas in the everyday practice of cytology are the following: mesothelioma vs. adenocarcinoma, neuroendocrine differentiation or not, the distinction of melanoma from adenocarcinoma and sarcoma, hepatocellular carcinoma vs. adenocarcinoma, and the origin of adenocarcinomas of unknown primary. The authors discuss how they approach these diagnostic problems in their everyday practice and how they incorporate EM in solving them.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012030867

Download citation: RISBibTeXText

PMID: 16316948

DOI: 10.1080/01913120500323415


Related references

The use of electron microscopy to refine diagnoses in the daily practice of cytopathology. Ultrastructural Pathology 28(2): 55-66, 2004

StatSTEM: An efficient approach for accurate and precise model-based quantification of atomic resolution electron microscopy images. Ultramicroscopy 171: 104-116, 2016

Meeting of the Swiss Society for Optics and Electron Microscopy, the Austrian Society for Electron Microscopy, 28th Meeting of the German Society for Electron Microscopy including workgroups on cryo-electron microscopy and scanning tunneling microscopy as well as the 30th Colloquium of the Workgroup for Electron Microscopy Imaging and Analysis of Surface Microscopy, Regensburg, Germany, September 8-12, 1997. European Journal of Cell Biology 74(Suppl. 45): 3-134, 1997

Scanning electron microscopy 1977 vol 1 proceedings of the 10th annual scanning electron microscope symposium and workshops on materials and component characterization quality control with the scanning electron microscopy scanning transmission electron microscopy scanning electron microscopy application to semi conductors analytical electron microscopy biological specimen preparation for scanning electron microscopy chicago illinois usa march 28 april 1 1977. Scanning Electron Microscopy 784, 1977

The utility of electron microscopy in cytopathology. Ultrastructural Pathology 34(5): 252-259, 2010

Precise method for identification and study of individual cells by light microscopy and scanning electron microscopy. Scanning 6(4): 183-186, 1984

Electron microscopy: the neglected tool of cytopathology. Diagnostic Cytopathology 8(3): Iv-Vi, 1992

A realistic approach to the use of electron microscopy and other ancillary diagnostic techniques in surgical pathology. American Journal of Surgical Pathology 19(3): 247-250, 1995

Cytopathology of toxic nephritis studied by electron microscopy. La Presse Medicale 65(76): 1685-1688, 1957

Cytopathology and scanning electron microscopy of the cervicovaginal microenvironment. Anatomical Record 214(3): 117A, 1986

Electron microscopy of some points of submicroscopic cytopathology of the renal tubes. Comptes Rendus des Seances de la Societe de Biologie et de Ses Filiales 151(2): 267-269, 1957

Scanning electron microscopy of alcohol-fixed cytopathology specimens. Acta Cytologica 25(5): 578-584, 1981

A correlative approach to colloidal gold labeling with video enhanced light microscopy low voltage scanning electron microscopy and high voltage electron microscopy. Hayat, M A (Ed ) Colloidal Gold, Vol 3 Principles, Methods, And Applications Xxiv+421p Academic Press, Inc : San Diego, California, Usa; London, England, Uk Illus 369-409, 1991

Scope of scanning electron microscopy, transmission electron microscopy and freeze fracture technique in diagnostic cytology of effusions. Scanning Electron Microscopy 1983(Pt 3): 1317-1327, 1983

Electron microscopy of the proventriculus in Marek's disease. Herpesvirus detection and cytopathology. Monatschefte fur Veterinarmedizin 27(Heft 17): 677-680, 1972