EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Enhancer-dependent inhibition of mouse renin transcription by inflammatory cytokines


Enhancer-dependent inhibition of mouse renin transcription by inflammatory cytokines



American Journal of Physiology. Renal Physiology 288(1): F117-F124



ISSN/ISBN: 1931-857X

PMID: 15367390

DOI: 10.1152/ajprenal.00333.2003

Inflammatory cytokines have been shown to inhibit renin gene expression in the kidney in vivo and the kidney tumor-derived As4.1 cell line. In this report, we show that cytokines oncostatin M (OSM), IL-6, and IL-1beta inhibit transcriptional activity associated with 4.1 kb of the mouse renin 5'-flanking sequence in As4.1 cells. The 242-bp enhancer (-2866 to -2625 bp) is sufficient to mediate the observed inhibitory effects. Sequences within the enhancer required for inhibition by each of these cytokines have been determined by deletional and mutational analysis. Results indicate that a 39-bp region (CEC) containing a cAMP-responsive element, an E-box, and a steroid receptor-binding site, previously identified as the most critical elements for enhancer activity, is sufficient for the inhibition induced by IL-1beta. However, mutation of each of the three component sites does not abolish the inhibition by IL-1beta, suggesting that the target(s) of cytokine action may not be the transcription factors binding directly to these sites. This CEC region is also critical, but not sufficient, for the inhibition mediated by OSM and IL-6. These data suggest that the direct target of the associated cytokines may be coactivators interacting with transcription factors binding at the enhancer. Finally, we show that OSM treatment caused a 17-fold increase in promoter activity when only 2,625 bp of the Ren-1(c) flanking sequence were tested, in which the enhancer is not present. Three regions including -2625 to -1217 bp, the HOX.PBX binding site at -60 bp, and -59 to +6 bp have been found to contribute to this induction.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012041474

Download citation: RISBibTeXText

Related references

The activity of the mouse renin promoter in cells that do not normally produce renin is dependent upon the presence of a functional enhancer. Febs Letters 255(2): 241-247, 1989

BET Inhibition Attenuates Helicobacter pylori-Induced Inflammatory Response by Suppressing Inflammatory Gene Transcription and Enhancer Activation. Journal of Immunology 196(10): 4132-4142, 2016

Homeostatic Response of Mouse renin Gene Transcription in a Hypertensive Environment Is Mediated by a Novel 5' Enhancer. Molecular and Cellular Biology 38(7), 2018

Inhibition of Inflammatory Gene Transcription by IL-10 Is Associated with Rapid Suppression of Lipopolysaccharide-Induced Enhancer Activation. Journal of Immunology 198(7): 2906-2915, 2017

The proximal portion of the mouse renin enhancer is required for full enhancer function in As41 cells. Hypertension 32(3): 626, 1998

The Th2 Cell Cytokines IL-4 and IL-13 Regulate Found in Inflammatory Zone 1/Resistin-Like Molecule a Gene Expression by a STAT6 and CCAAT/Enhancer-Binding Protein-Dependent Mechanism. Journal of Immunology 170(4): 89-96, 2003

The Th2 Cell Cytokines Il-4 and Il-13 Regulate Found in Inflammatory Zone 1/Resistin-Like Molecule Gene Expression by a Stat6 and Ccaat/Enhancer-Binding Protein-Dependent Mechanism. The Journal of Immunology 170(4): 1789-1796, 2003

Proximal portion of the mouse renin enhancer is required for transcriptional activation of renin promoter. FASEB Journal 12(8): A1393, 1998

The Th2 cell cytokines IL-4 and IL-13 regulate found in inflammatory zone 1/resistin-like molecule alpha gene expression by a STAT6 and CCAAT/enhancer-binding protein-dependent mechanism. Journal of Immunology 170(4): 1789-1796, 2003

The proximal portion of the mouse renin enhancer is required for transcriptional activation of the renin promoter in AS41 cells. FASEB Journal 12(4): A131, 1998

A mouse renin distal enhancer is essential for blood pressure homeostasis in BAC-rescued renin-null mutant mice. Journal of Receptor and Signal Transduction Research 34(5): 401-409, 2014

Transcription factor Sp1 inhibition, memory, and cytokines in a mouse model of Alzheimer's disease. American Journal of Neurodegenerative Disease 4(2): 40-48, 2015

Differential transcription of the mouse acute phase serum amyloid A genes in response to pro-inflammatory cytokines. Amyloid 9(4): 229-236, 2002

Differential transcription of the mouse acute phase serum amyloid A genes in response to pro-inflammatory cytokines. Amyloid: the International Journal of Experimental and Clinical Investigation: the Official Journal of the International Society of Amyloidosis 9(4): 229-236, 2002

Differential transcription of the human and mouse acute phase Serum Amyloid A genes in response to pro-inflammatory cytokines. Journal of Interferon and Cytokine Research 22(Suppl. 1): S-140, 2002