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Expression and characterization of antimicrobial peptide ABP-CM4 in methylotrophic yeast Pichia pastoris



Expression and characterization of antimicrobial peptide ABP-CM4 in methylotrophic yeast Pichia pastoris



Process Biochemistry 41(2): 251-256



Antibacterial peptides CM4 (ABP-CM4) is a linear cationic peptide that has antimicrobial properties. To explore a new approach of expression of ABP-CM4 in methylotrophic yeast Pichia pastoris, the gene of ABP-CM4 was obtained by recursive PCR (rPCR) and cloned into the vector pPICZaA. The Sacl-linearized plasmid pPICZaA-CM4 was transformed into R pastoris GS 115 by electroporation.

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Accession: 012071391

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DOI: 10.1016/j.procbio.2005.06.030



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