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Flow sorting of marine bacterioplankton after fluorescence in situ hybridization

Flow sorting of marine bacterioplankton after fluorescence in situ hybridization

Applied and Environmental Microbiology 70(10): 6210-6219

We describe an approach to sort cells from coastal North Sea bacterioplankton by How cytometry after in situ hybridization with rRNA-targeted horseradish peroxidase-labeled oligonucleotide probes and catalyzed fluorescent reporter deposition (CARD-FISH). In a sample from spring 2003 >90% of the cells were detected by CARD-FISH with a bacterial probe (EUB338).

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Accession: 012094858

Download citation: RISBibTeXText

PMID: 15466568

DOI: 10.1128/AEM.70.10.6210-6219.2004

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