+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Gene expression and regulation of nerve growth factor in atopic dermatitis mast cells and the human mast cell line-1

Gene expression and regulation of nerve growth factor in atopic dermatitis mast cells and the human mast cell line-1

Journal of Neuroimmunology 161(1-2): 87-92

The gene expression and regulation of nerve growth factor (NGF) in atopic dermatitis (AD) and the human mast cell line (HMC)-1 was investigated at the molecular level. NGF-stimulation of HMC-1 cells resulted in increases in tryptase activity and histamine contents, paralleled by an increase of tryptase and histamine at the transcriptional level. Also, an increased expression of NGF was found in AD lesions, in association with increased systemic NGF plasma levels. Further cutaneous sources for increased NGF levels were keratinocytes and fibroblasts. These findings demonstrate an increased expression of NGF in AD and effects on tryptase and histamine. Mast cells may be major mediators of neurotrophin effects in AD.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 012113717

Download citation: RISBibTeXText

PMID: 15748947

DOI: 10.1016/j.jneuroim.2004.12.019

Related references

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve-mast cell interaction in a hapten-induced mouse model of atopic dermatitis. British Journal of Dermatology 168(4): 771-778, 2013

BCL-2 and mast cell survival BCL-2 is overexpressed in a growth factor-independent mouse mast cell line but mast cells develop in BCL-2 null mice. FASEB Journal 8(4-5): A742, 1994

Dexamethasone suppresses gene expression and production of IL-13 by human mast cell line and lung mast cells. Journal Of Allergy & Clinical Immunology. 102(1): 134-142, Y, 1998

Mast cells and atopic dermatitis. Stereological quantification of mast cells in atopic dermatitis and normal human skin. Archives of Dermatological Research 289(5): 256-260, 1997

Synaptotagmin I expression in mast cells of normal human tissues, systemic mast cell disease, and a human mast cell leukemia cell line. Journal of Histochemistry and Cytochemistry 49(3): 341-346, 2001

Promotion of mouse fibroblast collagen gene expression by mast cells stimulated via the Fc epsilon RI. Role for mast cell-derived transforming growth factor beta and tumor necrosis factor alpha. Journal of Experimental Medicine 180(6): 2027-2037, 1994

Differential expression of mast cell growth factor receptor (c-kit) by peritoneal connective tissue-type mast cells and tissue culture-derived mast cells. Journal of Leukocyte Biology 55(5): 596-602, 1994

Up-regulation of mouse mast cell protease-6 gene by transforming growth factor-beta and activin in mast cell progenitors. Cellular Signalling 17(1): 121-128, 2004

Up-regulation of a novel gene transcript in PC12 cells stimulated with nerve growth factor and mast cells stimulated with stem cell factor. Society for Neuroscience Abstracts 22(1-3): 555, 1996

Expression of functional TrkA receptor tyrosine kinase in the HMC-1 human mast cell line and in human mast cells. Blood 90(5): 1807-1820, 1997

Synaptotagmin I expression in mast cells of normal and neoplastic human tissues, and human mast cell line, HMC-1. Pathology International 50(Supplement): A181, 2000

Differential expression of complement receptors on human basophils and mast cells. Evidence for mast cell heterogeneity and CD88/C5aR expression on skin mast cells. Journal of Immunology 155(6): 3152-3160, 1995

Regulation of epidermal cell growth by mast cell derived mediators in atopic dermatitis. Clinical Research 37(2): 699A, 1989

Regulation of mast cell characteristics by cytokines: divergent effects of interleukin-4 on immature mast cell lines versus mature human skin mast cells. Archives of Dermatological Research 296(3): 134-138, 2004

Nerve growth factor-beta induces mast-cell marker expression during in vitro culture of human umbilical cord blood cells. Immunology 99(3): 418-426, 2000