+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

High frequency shoot regeneration from Phyllanthus amarus Schum. & Thonn



High frequency shoot regeneration from Phyllanthus amarus Schum. & Thonn



Indian Journal of Biotechnology 3(1): 103-107



Shoot tip, nodal and internodal segments of Phyllanthus amarus Schum. & Thonn. were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzyl amino purine (BAP) and kinetin (Kn) with 15% coconut milk. All the explants responded for regeneration. High frequency (100%) and maximum number of shoots (18.30+-0.47) were recorded on medium containing BAP (0.5 mg/l) in shoot tip culture. In vitro shoots were excised from shoot clumps and transferred to rooting medium containing different concentrations of indole butyric acid (IBA) or naphthalene acetic acid (NAA). Maximum number of roots was induced in medium containing IBA (0.5 mg/l). Rooted plantlets were hardened on MS basal liquid medium and subsequently in sterile soil+vermiculite (1:1). Plantlets thus developed were successfully established and finally transferred to greenhouse. The survival rate of the plantlets in the field was found to be very high (85%).

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 012148133

Download citation: RISBibTeXText


Related references

Plant regeneration from alginate-encapsulated shoot tips of Phyllanthus amarus Schum and Thonn, a medicinally important plant species. In Vitro Cellular and Developmental Biology Plant 42(2): 109-113, 2006

Regeneration of plantlets from leaf and internode explants of Phyllanthus amarus Schum. and Thonn. African Journal of Biotechnology 8(10): 2209-2211, 2009

High frequency in vitro propagation of Phyllanthus amarus Schum. & Thom. by shoot tip culture. Indian Journal of Experimental Biology 39(11): 1184-1187, 2001

Toxicological assessment on extracts of Phyllanthus amarus Schum and Thonn. Scientific Research And Essays: 9, 210-215, 2008

Physicochemical characterization of phyllanthin from Phyllanthus amarus Schum. et Thonn. Drug Development and Industrial Pharmacy 40(6): 793-802, 2014

Antibacterial activity of Phyllanthus amarus Schum and Thonn on five vegetative organisms. Plant Archives: 2, 563-568, 2008

Phyllanthin biosynthesis in Phyllanthus amarus Schum and Thonn growing at different altitudes. Journal Of Medicinal Plants Research: 1, 41-48, 2010

Development of methods for isolation and purification of phyllanthin from Phyllanthus amarus Schum and Thonn. Phytomedicine 7(Suppl. 2): 81, 2000

Supercritical fluid extraction of phyllanthin and niranthin from Phyllanthus amarus Schum. & Thonn. Journal of Supercritical Fluids 127: 23-32, 2017

Anti-mutagenic activity of Phyllanthus amarus Schum & Thonn in vitro as well as in vivo. Teratogenesis, Carcinogenesis, and Mutagenesis 22(4): 285-291, 2002

The effects of chromatographic fractions of Phyllanthus amarus Schum. et Thonn. On the haematological parameters of rats. Folia Veterinaria 49(2): 65-69, 2005

The quantification of ellagic acid in the crude extract of Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae). Phytochemical Analysis 22(4): 361-366, 2011

Effect of Phyllanthus amarus Schum. and Thonn. and its protective mechanism on paracetamol hepatotoxicity in rats. Acta Horticulturae (680): 195-201, 2005

Hypotensive effect of aqueous extract of the leaves of Phyllanthus amarus Schum and Thonn (Euphorbiaceae). Acta Poloniae Pharmaceutica 64(6): 547-552, 2008

Effect of Phyllanthus amarus Schum. & Thonn. and its protective mechanism on paracetamol hepatotoxicity in rats. Acta Horticulturae 680: 95-201, 2005