Section 13
Chapter 12,159

Human E2F6 is alternatively spliced to generate multiple protein isoforms

Kherrouche, Z.; De Launoit, Y.; Monté, D.

Biochemical and Biophysical Research Communications 317(3): 749-760


ISSN/ISBN: 0006-291X
PMID: 15081404
DOI: 10.1016/j.bbrc.2004.03.099
Accession: 012158413

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E2F6 protein belongs to the family of the E2F transcription factors. Here, we showed that the human E2F6 gene contains nine exons distributed along 20.4kbp of genomic DNA on chromosome 2 leading to the transcription of six alternatively spliced E2F6 mRNAs that encode four different E2F6 proteins. Moreover, we identified an E2F6 pseudogene localized on chromosome 22 completely spliced and devoid of exons 2, 3, and 4, and part of exons 1 and 5. Definition of the transcriptional initiation site and sequence analysis show that the gene contains a TATA less, CAAT less, GC-rich promoter with multiple transcription start sites. Regulatory elements necessary for basal transcription reside within a 134bp fragment as determined by transient transfection experiments.

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