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Immunohistochemistry and c-kit gene analysis in determining malignancy in gastrointestinal stromal tumors

Immunohistochemistry and c-kit gene analysis in determining malignancy in gastrointestinal stromal tumors

Hepato-Gastroenterology 50(53): 1431-1435

Background/Aims: Usefulness of immunohistochemistry and c-kit proto-oncogene mutation was examined for determining malignancy in 24 cases of gastrointestinal stromal tumors. Methodology: Cases were histologically diagnosed and subjected to immunohistochemical staining and c-kit gene analysis. All parameters were compared to prognosis. Results: There were significant differences in tumor size, central necrosis, mitotic activity and histological diagnosis between recurrent and non-recurrent cases. Positivity to KIT staining was 100% in recurrent and 87.5% in non-recurrent cases. Positivity to Ki-67 and p53 staining were significantly higher in recurrent cases than in non-recurrent cases. Mutations in exon 11 of the c-kit gene were significantly more frequent in recurrent cases than in non-recurrent cases. Conclusions: Histological diagnosis, tumor size, central necrosis, and mitotic activity were reconfirmed to be indicators for recurrence of gastrointestinal stromal tumors. Furthermore, it is suggested that positivity of Ki-67 and p53 immunostaining and c-kit gene mutation also need to be done for prediction of recurrence.

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Accession: 012180611

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PMID: 14571755

DOI: 10.1016/s0016-5085(03)83315-2

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