Improving nature's enzyme active site with genetically encoded unnatural amino acids
Jackson, J.C.; Duffy, S.P.; Hess, K.R.; Mehl, R.A.
Journal of the American Chemical Society 128(34): 11124-11127
The ability to site-specifically incorporate a diverse set of unnatural amino acids (> 30) into proteins and quickly add new structures of interest has recently changed our approach to protein use and study. One important question yet unaddressed with unnatural amino acids ( UAAs) is whether they can improve the activity of an enzyme beyond that available from the natural 20 amino acids.