Home
  >  
Section 13
  >  
Chapter 12,190

Improving nature's enzyme active site with genetically encoded unnatural amino acids

Jackson, J.C.; Duffy, S.P.; Hess, K.R.; Mehl, R.A.

Journal of the American Chemical Society 128(34): 11124-11127

2006

ISSN/ISBN: 0002-7863
PMID: 16925430
DOI: 10.1021/ja061099y
Accession: 012189267
Download citation:  
Text
  |  
BibTeX
  |  
RIS
The ability to site-specifically incorporate a diverse set of unnatural amino acids (> 30) into proteins and quickly add new structures of interest has recently changed our approach to protein use and study. One important question yet unaddressed with unnatural amino acids ( UAAs) is whether they can improve the activity of an enzyme beyond that available from the natural 20 amino acids.

PDF emailed within 0-6 h: $19.90




Related References

Hu, C.; Wang, J. 2016: Method for Enzyme Design with Genetically Encoded Unnatural Amino Acids Methods in Enzymology 580: 109-133
Thielges, M.C.; Axup, J.Y.; Wong, D.; Lee, H.S.; Chung, J.K.; Schultz, P.G.; Fayer, M.D. 2011: Two-dimensional IR spectroscopy of protein dynamics using two vibrational labels: a site-specific genetically encoded unnatural amino acid and an active site ligand Journal of Physical Chemistry. B 115(38): 11294-11304
Ye, S; Kazmi, M A.; Duarte, T; Naganathan, S; Sakmar, T P.; Huber, T 2010: Site-Specific Fluorescent Labeling of Purified G-Protein-Coupled Receptors Using Genetically-Encoded Unnatural Amino Acids Biophysical Journal 98(3): 291a-292a
Kim, C.H.; Axup, J.Y.; Schultz, P.G. 2013: Protein conjugation with genetically encoded unnatural amino acids Current Opinion in Chemical Biology 17(3): 412-419
Klippenstein, V.; Ghisi, V.; Wietstruk, M.; Plested, A.J.R. 2014: Photoinactivation of glutamate receptors by genetically encoded unnatural amino acids Journal of Neuroscience: the Official Journal of the Society for Neuroscience 34(3): 980-991
Kim, C.H.; Axup, J.Y.; Dubrovska, A.; Kazane, S.A.; Hutchins, B.A.; Wold, E.D.; Smider, V.V.; Schultz, P.G. 2012: Synthesis of bispecific antibodies using genetically encoded unnatural amino acids Journal of the American Chemical Society 134(24): 9918-9921
Fleissner, M.R.; Brustad, E.M.; Kálai, T.ás.; Altenbach, C.; Cascio, D.; Peters, F.B.; Hideg, K.ál.án.; Peuker, S.; Schultz, P.G.; Hubbell, W.L. 2009: Site-directed spin labeling of a genetically encoded unnatural amino acid Proceedings of the National Academy of Sciences of the United States of America 106(51): 21637-21642
Kaya, E.; Gutsmiedl, K.; Vrabel, M.; Müller, M.; Thumbs, P.; Carell, T. 2009: Synthesis of threefold glycosylated proteins using click chemistry and genetically encoded unnatural amino acids Chembiochem: a European Journal of Chemical Biology 10(18): 2858-2861
Li, M.; Wang, F.; Yan, L.; Lu, M.; Zhang, Y.; Peng, T. 2022: Genetically encoded fluorescent unnatural amino acids and FRET probes for detecting deubiquitinase activities Chemical Communications 58(73): 10186-10189
Frost, J.R.; Jacob, N.T.; Papa, L.J.; Owens, A.E.; Fasan, R. 2015: Ribosomal Synthesis of Macrocyclic Peptides in Vitro and in Vivo Mediated by Genetically Encoded Aminothiol Unnatural Amino Acids Acs Chemical Biology 10(8): 1805-1816
Naganathan, S.; Ye, S.; Sakmar, T.P.; Huber, T. 2013: Site-specific epitope tagging of G protein-coupled receptors by bioorthogonal modification of a genetically encoded unnatural amino acid Biochemistry 52(6): 1028-1036
Naganathan, S.; Ye, S.; Sakmar, T.P.; Huber, T. 2013: Site-specific epitope tagging of G protein-coupled receptors by bioorthogonal modification of a genetically encoded unnatural amino acid Biochemistry 52(6): 1028-1036
Miyake-Stoner, S.J.; Miller, A.M.; Hammill, J.T.; Peeler, J.C.; Hess, K.R.; Mehl, R.A.; Brewer, S.H. 2009: Probing protein folding using site-specifically encoded unnatural amino acids as FRET donors with tryptophan Biochemistry 48(25): 5953-5962
Shi, P.; Xi, Z.; Wang, H.; Shi, C.; Xiong, Y.; Tian, C. 2010: Site-specific protein backbone and side-chain NMR chemical shift and relaxation analysis of human vinexin SH3 domain using a genetically encoded 15N/19F-labeled unnatural amino acid Biochemical and Biophysical Research Communications 402(3): 461-466
Ugwumba, I.N.; Ozawa, K.; Xu, Z.-Q.; Ely, F.; Foo, J.-L.; Herlt, A.J.; Coppin, C.; Brown, S.; Taylor, M.C.; Ollis, D.L.; Mander, L.N.; Schenk, G.; Dixon, N.E.; Otting, G.; Oakeshott, J.G.; Jackson, C.J. 2011: Improving a natural enzyme activity through incorporation of unnatural amino acids Journal of the American Chemical Society 133(2): 326-333
Zheng, S.; Kwon, I. 2013: Controlling enzyme inhibition using an expanded set of genetically encoded amino acids Biotechnology and Bioengineering 110(9): 2361-2370
Wang, L.; Zhang, J.; Han, M.-J.; Zhang, L.; Chen, C.; Huang, A.; Xie, R.; Wang, G.; Zhu, J.; Wang, Y.; Liu, X.; Zhuang, W.; Li, Y.; Wang, J. 2021: A Genetically Encoded Two-Dimensional Infrared Probe for Enzyme Active-Site Dynamics Angewandte Chemie 60(20): 11143-11147
Jones, D.H.; Cellitti, S.E.; Hao, X.; Zhang, Q.; Jahnz, M.; Summerer, D.; Schultz, P.G.; Uno, T.; Geierstanger, B.H. 2010: Site-specific labeling of proteins with NMR-active unnatural amino acids Journal of Biomolecular NMR 46(1): 89-100
Dawadi, P.B.S.; Lugtenburg, J. 2013: Access to any site directed stable isotope ((2)H, (13)C, (15)N, (17)O and (18)O) in genetically encoded amino acids Molecules 18(1): 482-519
Loh, C.T.; Adams, L.A.; Graham, B.; Otting, G. 2018: Genetically encoded amino acids with tert-butyl and trimethylsilyl groups for site-selective studies of proteins by NMR spectroscopy Journal of Biomolecular NMR 71(4): 287-293