Section 13
Chapter 12,199

Increased expression of polypyrimidine tract binding protein results in higher insulin mRNA levels

Fred, R.G.; Welsh, N.

Biochemical and Biophysical Research Communications 328(1): 38-42


ISSN/ISBN: 0006-291X
PMID: 15670747
DOI: 10.1016/j.bbrc.2004.12.147
Accession: 012198964

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The aim of this study was to further elucidate the role of the polypyrimidine tract binding protein (PTB) in the control of insulin mRNA stability. We observed that the glucose- or interleukin-1beta-induced increase in insulin mRNA was paralleled by an increase in PTB mRNA. To further test the hypothesis that PTB controls insulin gene expression, betaTC-6 cells were treated with a PTB-specific siRNA to modify the beta-cell content of PTB. Surprisingly, we observed an increase in PTB mRNA and PTB protein levels in response to the siRNA treatment. In addition, the PTB-siRNA treatment also increased insulin mRNA. We conclude that expression of the PTB gene controls insulin production.

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