+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Induction of Ym1/2 in mouse bone marrow-derived mast cells by IL-4 and identification of Ym1/2 in connective tissue type-like mast cells derived from bone marrow cells cultured with IL-4 and stem cell factor



Induction of Ym1/2 in mouse bone marrow-derived mast cells by IL-4 and identification of Ym1/2 in connective tissue type-like mast cells derived from bone marrow cells cultured with IL-4 and stem cell factor



Immunology and Cell Biology 83(5): 468-474



Mast cells play an important role in allergic inflammation by releasing various bioactive mediators. The function of mast cells is enhanced by various stimuli, partly due to the induction of specific genes and their products. Although many inducible genes have been identified, a significant number of genes remain to be identified. Therefore, this study used PCR-selected cDNA subtraction to establish the profile of induced genes in the connective tissue (CT) type-like mast cells derived from bone marrow cells cultured in the presence of IL-4 and stem cell factor. Two hundred and fifty cDNA clones were obtained from the CT type-like mast cells by PCR-selected cDNA subtraction. Among them, Ym1/2, a chitinase-like protein, is one of the most abundantly induced genes. Ym1 is produced by activated macrophages in a parasitic infection, whereas its isotype, Ym2, is highly upregulated in allergic lung disease. In order to differentiate which isotype is expressed in bone marrow cells, specific primers for bone marrow-derived mast cells (BMMC), and CT type-like mast cells were used for RT-PCR. The results showed that Ym1 was constitutively expressed in bone marrow cells and gradually decreased in the presence of IL-3, whereas Ym2 was induced only in the presence of IL-4. CT type-like mast cells from bone marrow cells expressed Ym1 throughout the culture period and Ym2 was induced only by the addition of IL-4 into BMMC, indicating that IL-4 is essential for the expression of Ym1/2 genes.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012203407

Download citation: RISBibTeXText

PMID: 16174095

DOI: 10.1111/j.1440-1711.2005.01352.x


Related references

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell- deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells. Journal of Experimental Medicine 162(3): 1025-1043, 1985

Fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient W/Wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells. Journal of Experimental Medicine 162(3): 1025-1043, 1985

Pillars article: fate of bone marrow-derived cultured mast cells after intracutaneous, intraperitoneal, and intravenous transfer into genetically mast cell-deficient w/wv mice. Evidence that cultured mast cells can give rise to both connective tissue type and mucosal mast cells. Journal of Immunology 183(11): 6863-6881, 2009

Changing processes from bone marrow-derived cultured mast cells to connective tissue-type mast cells in the peritoneal cavity of mast cell-deficient w/wv mice: association of proliferation arrest and differentiation. Journal of Immunology 138(2): 544-549, 1987

TLR3-, TLR7-, and TLR9-mediated production of proinflammatory cytokines and chemokines from murine connective tissue type skin-derived mast cells but not from bone marrow-derived mast cells. Journal of Immunology 173(1): 531-541, 2004

Rat bone marrow-derived mast cells co-cultured with fibroblasts in the absence of T cell-derived cytokines are stem cell factor-dependent for their survival and maintain their mucosal mast cell-like phenotype. 9TH INTERNATIONAL CONGRESS OF IMMUNOLOGY [Author] The 9th International Congress of Immunology : 39, 1995

Rat bone marrow-derived mast cells co-cultured with 3T3 fibroblasts in the absence of T-cell derived cytokines require stem cell factor for their survival and maintain their mucosal mast cell-like phenotype. Immunology 88(3): 375-383, 1996

In vitro growth of normal bone marrow derived human basophils and the comparison of the cells to bone marrow derived mouse mast cell like cells. Immunobiology 163(2-4): 272-273, 1982

Stem cell factor and interleukin-4 induce murine bone marrow cells to develop into mast cells with connective tissue type characteristics in vitro. Experimental Hematology 27(4): 654-662, 1999

Cloned mouse mast cells derived from immunized lymph node cells and from foetal liver cells exhibit characteristics of bone marrow-derived mast cells containing chondroitin sulphate E proteoglycan. Immunology 52(3): 563-575, 1984

Cloned mouse mast cells derived from immunized lymph node cells and from fetal liver cells exhibit characteristics of bone marrow derived mast cells containing chondroitin sulfate e proteo glycan. Immunology 52(3): 563-576, 1984

Mast cell heterogeneity. Differential synthesis and expression of glycosphingolipids by mouse serosal mast cells as compared to IL-3-dependent bone marrow culture-derived mast cells before or after coculture with 3T3 fibroblasts. Journal of Immunology 145(5): 1463-1468, 1990

Stem cell factor enhances immunoglobulin E-dependent mediator release from cultured rat bone marrow-derived mast cells: Activation of previously unresponsive cells demonstrated by a novel ELISPOT assay. Immunology 87(2): 326-333, 1996

Early expression of FcepsilonRI in and on human bone marrow-derived mast cells cultured in the presence of stem cell factor and interleukin-6. FASEB Journal 15(5): A1019, 2001

Mouse bone-marrow progenitors differentiate into eosinophils when cultured in conditioned media obtained from activated mouse bone-marrow derived mast cells. Journal of Allergy & Clinical Immunology 91(1 Part 2): 175, 1993