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Insights into the mechanism and catalysis of the native chemical ligation reaction



Insights into the mechanism and catalysis of the native chemical ligation reaction



Journal of the American Chemical Society 128(20): 6640-6646



Native chemical ligation of unprotected peptide segments involves reaction between a peptide-alpha-thioester and a cysteine-peptide, to yield a product with a native amide bond at the ligation site. Peptide-alpha-thioalkyl esters are commonly used because of their ease of preparation. These thioalkyl esters are rather unreactive so the ligation reaction is catalyzed by in situ transthioesterification with thiol additives.

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Accession: 012220202

Download citation: RISBibTeXText

PMID: 16704265

DOI: 10.1021/ja058344i


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