EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Intragranulomatous necrosis in lungs of mice infected by aerosol with Mycobacterium tuberculosis is related to bacterial load rather than to any one cytokine or T cell type



Intragranulomatous necrosis in lungs of mice infected by aerosol with Mycobacterium tuberculosis is related to bacterial load rather than to any one cytokine or T cell type



Microbes and Infection 8(3): 628-636



Low dose aerosol infection of C5713L/6 mice with a clinical strain of Mycobacterium tuberculosis (UTE 0335 R) induced intragranulomatous necrosis in pulmonary granulomas (INPG) at week 9 postinfection. Infection of different knockout (KO) mouse strains with UTE 0335 R induced INPG in all strains and established two histopathological patterns. The first pattern was seen in SCID mice and in mice with deleted alpha/beta T receptor, TNF R1, IL-12, IFN-gamma, or NOS genes, and showed a massive INPG with a high granulomatous infiltration of the lung, a large and homogeneous eosinophilic necrosis full of acid-fast bacilli, with marked karyorrhexis, coarse basophilic necrosis, and surrounded by patches delimited by partially conserved alveolar septum full of PMNs. The second pattern was seen in mice with deleted IL-1 R1, IL-6, IL-10, CD4, CD8 or gamma/delta T cell receptor genes, and showed more discrete lesions with predominant homogeneous eosinophilic necrosis with few bacilli and surrounded by a well-defined lymphocyte-based ring. Local expression of IFN-gamma, NOS, TNF and RANTES showed no significant differences between these mouse strains generating a discrete INPG. Mouse strains showing a massive INPG showed higher, lower or equal expression values compared to the control strain. In conclusion, the severity of the INPG pattern correlated with pulmonary CFU counts, irrespective of the genetic absence or the infection-induced levels of cytokine mediators. (c) 2006 Elsevier SAS. All rights reserved.

(PDF emailed within 0-6 h: $19.90)

Accession: 012231710

Download citation: RISBibTeXText

PMID: 16515876

DOI: 10.1016/j.micinf.2005.08.014



Related references

Intragranulomatous necrosis in pulmonary granulomas is not related to resistance against Mycobacterium tuberculosis infection in experimental murine models induced by aerosol. International Journal of Experimental Pathology 87(2): 139-149, 2006

Towards a 'human-like' model of tuberculosis: Intranasal inoculation of LPS induces intragranulomatous lung necrosis in mice infected aerogenically with Mycobacterium tuberculosis. Scandinavian Journal of Immunology 53(1): 65-71, January, 2001

Deletion of Mycobacterium tuberculosis sigma factor E results in delayed time to death with bacterial persistence in the lungs of aerosol-infected mice. Infection and Immunity 71(12): 7170-7172, 2003

Immunization of mice with a Mycobacterium tuberculosis genomic expression library results in lower bacterial load in lungs after challenge with BCG. Tuberculosis 86(3-4): 247-254, 2006

Early emergence of CD8(+) T cells primed for production of type 1 cytokines in the lungs of Mycobacterium tuberculosis-infected mice. Infection and Immunity 67(8): 3980-3988, 1999

Stable T-cell population expressing an effector cell surface phenotype in the lungs of mice chronically infected with Mycobacterium tuberculosis. Infection and Immunity 72(1): 570-575, 2003

Mycobacterium tuberculosis can gain access to adipose depots of mice infected via the intra-nasal route and to lungs of mice with an infected subcutaneous fat implant. Microbial Pathogenesis 93: 32-37, 2016

CD8+ CTL from lungs of Mycobacterium tuberculosis-infected mice express perforin in vivo and lyse infected macrophages. Journal of Immunology 165(1): 353-363, 2000

Antibody response to phenolic glycolipid i and mycobacterium w antigens and its relation to bacterial load in mycobacterium leprae infected mice and leprosy patients. Clinical & Experimental Immunology 78(2): 214-218, 1989

Evolution of granulomas in lungs of mice infected aerogenically with Mycobacterium tuberculosis. Scandinavian Journal of Immunology 52(2): 156-163, 2000

MicroRNA expression signatures in lungs of mice infected with Mycobacterium tuberculosis. Tuberculosis 101(): 151-159, 2016

Immunohistochemical study of granuloma formation in the lungs of mice infected with Mycobacterium tuberculosis. Abstracts of the General Meeting of the American Society for Microbiology 101: 332, 2001

Influence of H-2 genes on growth of Mycobacterium tuberculosis in the lungs of chronically infected mice. Immunology 76(1): 129-132, 1992