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Isolation and purification of phycoerythrin from Porphyridium cruentum and its spectrum characteristics



Isolation and purification of phycoerythrin from Porphyridium cruentum and its spectrum characteristics



Journal of Plant Resources and Environment 15(2): 20-24



The freeze dried powder of Porphyridium cruentum Naegeli was extracted with distillated water at 4 degrees C, centrifugilized, precipitated by (NH4)(2)SO4, chromatographed on a DEAE-Sepharose Fast Flow ion exchange column. As a result, the B-phycoerythrin was got with a purity of 4.85 and a yield of 51.9%, and with a purity of 5.10 and a yield of 34.0% when it was further purified by hydroxyl column chromatography.

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