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Large-scale identification of expressed sequence tags (ESTs) from Nicotiana tabacum by normalized cDNA library sequencing



Large-scale identification of expressed sequence tags (ESTs) from Nicotiana tabacum by normalized cDNA library sequencing



Beitraege zur Tabakforschung International 22(2): 114-124



An expressed sequence tags (EST) resource for tobacco plants (Nicotiana tabacum) was established using high-throughput sequencing of randomly selected clones from one cDNA library representing a range of plant organs (leaf, stem, root and root base). Over 5000 ESTs were generated from the 3' ends of 8000 clones, analyzed by BLAST searches and categorized functionally.

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Accession: 012256298

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