+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Monoclonal anti-leukemia inhibitory factor antibody inhibits blastocyst implantation in the rhesus monkey



Monoclonal anti-leukemia inhibitory factor antibody inhibits blastocyst implantation in the rhesus monkey



Contraception 74(5): 419-425



Leukemia inhibitory factor (LIF) appears important in the process of blastocyst implantation in primates. In the present study, we investigated the effect on pregnancy outcome of the administration of a monoclonal antibody (mAb) to recombinant human (rh) LIF (anti-LIF mAb) into the uterine cavity of mated female rhesus monkeys during the peri-implantation period. Two milligrams of either mouse mAb to rhLIF or isotype-matched immunoglobulin (Ig) was administered into the uterine cavity on estimated Day 5 or Day 8 after ovulation either through the vagina (n=33) or through the oviduct (n=29) of successfully mated females. There was a significant (p<.04) decline in pregnancy outcome in groups treated with anti-LIF mAb (8 pregnancies from 32 animals) compared with control animals (24 pregnancies from 30 animals). There was, however, no significant difference in pregnancy inhibition between a group of animals subjected to vaginal route treatment and a group of animals subjected to oviductal route treatment, as well as between a group subjected to anti-LIF mAb on Day 5 after ovulation and a group subjected to anti-LIF mAb on Day 8 after ovulation. No significant change was seen in the number of viable pregnancy in animals treated with 6 mg of anti-LIF mAb (5 pregnancies from 16 animals) compared with animals treated with 2 mg of anti-LIF mAb (8 pregnancies from 32 animals). Serum profiles of estradiol, progesterone, monkey chorionic gonadotropin and mouse IgG did not show any difference among different treatment subgroups during the luteal phase. However, among animals treated with anti-LIF mAb, the mean area under the curve for serum mouse IgG in pregnant animals (234+/-55 microg/mL day) was significantly (p<.01) less than that of nonpregnant animals (1325+/-97 microg/mL day). The results of the present study put forward the proof of concept that LIF plays a critical role in the process of blastocyst implantation in the rhesus monkey.

(PDF emailed within 0-6 h: $19.90)

Accession: 012325349

Download citation: RISBibTeXText

PMID: 17046385

DOI: 10.1016/j.contraception.2006.05.070


Related references

Polyethylene glycated leukemia inhibitory factor antagonist inhibits human blastocyst implantation and triggers apoptosis by down-regulating embryonic AKT. Fertility and Sterility 100(4): 1160-1169, 2014

Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey. Cytokine 24(6): 277-285, 2003

Immunohistochemical localization of leukemia inhibitory factor, interleukins 1alpha and 1beta, and IL-6 in embryo-endometrium interface during implantation in the rhesus monkey. Developmental Biology 235(1): 182, July 1, 2001

Effect of vaginally administered fumagillin on immunohistochemical distribution of leukemia inhibitory factor, interleukin 6, transforming growth factor beta and vascular endothelial growth factor in implantation stage endometrium of the rhesus monkey. Indian Journal of Physiology and Pharmacology 46(1): 25-35, 2002

Leukemia inhibitory factor, leukemia inhibitory factor receptor, and glycoprotein 130 in rhesus monkey uterus during menstrual cycle and early pregnancy. Biology of Reproduction 63(2): 508-512, 2000

The chimeric monoclonal antibody MHCSZ-123 against human von Willebrand factor A3 domain inhibits high-shear arterial thrombosis in a Rhesus monkey model. Journal of Hematology and Oncology 10(1): 111, 2018

In vivo effect of leukemia inhibitory factor (LIF) and an anti-LIF polyclonal antibody on murine embryo and fetal development following exposure at the time of transcervical blastocyst transfer. Biology of Reproduction 67(2): 460-464, 2002

A humanized anti-tissue factor monoclonal antibody inhibits procoagulant effects of LPS challenge in a cynomolgus monkey model. Inflammation Research 52(Supplement 2): S 136, July, 2003

Leukemia inhibitory factor and its physiological functions during embryo development and blastocyst implantation. Sheng Li Ke Xue Jin Zhan 28(3): 240-242, 1997

Uterine expression of leukemia inhibitory factor coincides with the onset of blastocyst implantation. Proceedings of the National Academy of Sciences of the United States of America 88(24): 11408-11412, 1991

Expression of leukemia inhibitory factor lif in the uterus coincides with the onset of implantation of the blastocyst. Journal of Cellular Biochemistry Supplement (16 PART F): 93, 1992

Leukemia inhibitory factor and IL-6 detection in porcine uterine secretions prior to blastocyst implantation. Lymphokine & Cytokine Research 12(5): 392, 1993

Blastocyst-endometrium interaction at implantation in the rhesus monkey. Journal of Reproductive Immunology 53(1-2): 227-239, 2001

Nitric oxide in blastocyst implantation in the rhesus monkey. Reproduction 130(3): 321-332, 2005

Evidence for heterodimeric association of leukemia inhibitory factor (LIF) receptor and gp130 in the mouse uterus for LIF signaling during blastocyst implantation. Reproduction 131(2): 341-349, 2006