+ Translate
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Natural killer cell-mediated rejection of experimental human lung cancer by genetic overexpression of major histocompatibility complex class i chain-related gene a

Natural killer cell-mediated rejection of experimental human lung cancer by genetic overexpression of major histocompatibility complex class i chain-related gene a

Human Gene Therapy 17(2): 135-146

ISSN/ISBN: 1043-0342

PMID: 16454647

DOI: 10.1089/hum.2006.17.135

Studies have highlighted molecular and cell biological mechanisms of the NKG2D-NKG2D ligand system in the activation of natural killer (NK) cell and T cell functions. In this study we explore the potential of genetic overexpression of human major histocompatibility complex class I chain-related gene A (MICA), a powerful NKG2D ligand, for the induction of NK cell-mediated antitumor immunity in a humanized murine model of non-small cell lung cancer. Using tissue microarray technology we detected expression of MICA in only 30% of the samples from patients with lung cancer. Staining was always weak and focal, indicating that expression of MICA is detectable but limited in human lung cancer. Genetic overexpression of MICA by means of adenoviral transduction or transfection of expression plasmids was feasible in cell lines in vitro, primary human cancer tissue ex vivo, and in experimental human cancers in vivo. The presence of MICA on the surface of largely NK cell-resistant human lung cancer cells reestablished NK cell susceptibility and provoked NK cell-mediated antitumor immunity by murine and human NK cells in two different experimental therapy models. In this study we analyze the interaction of human NK cells with MICA-positive human cancer cells in an in vivo setting. Our data demonstrate that MICA overexpression can function as NK cell-mediated immunotherapy in experimental lung cancer.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 012343112

Download citation: RISBibTeXText

Related references

Interferon-γ up-regulates major-histocompatibility-complex class I-related chain a expression and enhances major-histocompatibility-complex class I-related chain A-mediated cytolysis of human corneal epithelium by natural killer cells in vitro. Journal of Interferon and Cytokine Research: the Official Journal of the International Society for Interferon and Cytokine Research 32(3): 115-120, 2012

Associations of MICB with cervical cancer in north-eastern Thais: identification of major histocompatibility complex class I chain-related gene B motifs influencing natural killer cell activation. Clinical and Experimental Immunology 153(2): 205-213, 2008

Natural killer group 2 member D cell recruitment driven by major histocompatibility complex class I chain-related antigens A and B: a possible mechanism during acute intestinal allograft rejection in the mouse. Transplantation Proceedings 42(10): 4467-4469, 2010

Inhibition of natural killer cell-mediated bone marrow graft rejection by allogeneic major histocompatibility complex class I, but not class Ii molecules. European Journal of Immunology 25(5): 1286-1291, 1995

M144, a murine cytomegalovirus (MCMV)-encoded major histocompatibility complex class I homologue, confers tumor resistance to natural killer cell-mediated rejection. Journal of Experimental Medicine 190(3): 435-444, 1999

Natural killer and cytotoxic T lymphocyte-mediated cytotoxicity enhanced by genetic overexpression of MHC class i chain-related protein a in oral squamous cell carcinoma: an experimental study in vivo. Hua Xi Kou Qiang Yi Xue Za Zhi 30(1): 32-35, 2012

Upregulated mRNA expression of major histocompatibility complex class i chain-related gene a in colon and activated natural killer cells of Chinese patients with ulcerative colitis. Journal of Digestive Diseases 12(2): 82-89, 2011

Genetic defects of the IRF1-mediated major histocompatibility complex class I antigen presentation pathway occur prevalently in the JAK2 gene in non-small cell lung cancer. Oncotarget 8(37): 60975-60986, 2017

The human natural killer cell receptor for major histocompatibility complex class i molecules. Surface modulation of p58 molecules and their linkage to CD3 zeta chain, Fc epsilon Ri gamma chain and the p56lck kinase. European Journal of Immunology 24(10): 2527-2534, 1994

Major histocompatibility complex class I-related chain a and UL16-binding protein expression on tumor cell lines of different histotypes: analysis of tumor susceptibility to NKG2D-dependent natural killer cell cytotoxicity. Cancer Research 62(21): 6178-6186, 2002

Lack and restoration of sensitivity of lung cancer cells to cellular attack with special reference to expression of human leukocyte antigen class I and/or major histocompatibility complex class I chain related molecules A/B. Cancer Science 98(11): 1795-1802, 2007

Ly-49-independent inhibition of natural killer cell-mediated cytotoxicity by a soluble major histocompatibility complex class i molecule. European Journal of Immunology 24(9): 2110-2114, 1994

Natural killer cell receptors for major histocompatibility complex class i and related molecules in cytomegalovirus infection. Tissue Antigens 63(3): 195-203, 2004

Staphylococcus-mediated T-cell activation and spontaneous natural killer cell activity in the absence of major histocompatibility complex class II molecules. Infection and Immunity 61(9): 4013-4016, 1993

Human T-cell leukemia virus type 1 (HTLV-1) p12I down-modulates ICAM-1 and -2 and reduces adherence of natural killer cells, thereby protecting HTLV-1-infected primary CD4+ T cells from autologous natural killer cell-mediated cytotoxicity despite the reduction of major histocompatibility complex class I molecules on infected cells. Journal of Virology 81(18): 9707-9717, 2007