Neuron-restrictive silencer factor (NRSF) functions as a repressor in neuronal cells to regulate the mu opioid receptor gene
Kim, C.Sung.; Hwang, C.Kyu.; Choi, H.Sun.; Song, K.Young.; Law, P-Yee.; Wei, L-Na.; Loh, H.H.
Journal of Biological Chemistry 279(45): 46464-46473
ISSN/ISBN: 0021-9258 PMID: 15322094 DOI: 10.1074/jbc.m403633200
The mu opioid receptor (MOR) is expressed in the central nervous system and specific cell lines with varying expression levels perhaps playing important roles. One of the neuronal-specific transcription regulators, neuron-restrictive silencer factor ( NRSF), has been shown to repress the expression of neuron-specific genes in non-neuronal cells. However, we showed here that the neuron-restrictive silencer element (NRSE) of MOR functions as a critical regulator to repress the MOR gene expression in specific neuronal cells depending on NRSF expression level. Using co-transfection studies, we showed that the NRSE of the MOR promoter is functional in NRSF-positive cells (NS20Y and HeLa) but not in NRSF-negative cells (PC12).