Section 13
Chapter 12,349

Neuron-restrictive silencer factor (NRSF) functions as a repressor in neuronal cells to regulate the mu opioid receptor gene

Kim, C.Sung.; Hwang, C.Kyu.; Choi, H.Sun.; Song, K.Young.; Law, P-Yee.; Wei, L-Na.; Loh, H.H.

Journal of Biological Chemistry 279(45): 46464-46473


ISSN/ISBN: 0021-9258
PMID: 15322094
DOI: 10.1074/jbc.m403633200
Accession: 012348580

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The mu opioid receptor (MOR) is expressed in the central nervous system and specific cell lines with varying expression levels perhaps playing important roles. One of the neuronal-specific transcription regulators, neuron-restrictive silencer factor ( NRSF), has been shown to repress the expression of neuron-specific genes in non-neuronal cells. However, we showed here that the neuron-restrictive silencer element (NRSE) of MOR functions as a critical regulator to repress the MOR gene expression in specific neuronal cells depending on NRSF expression level. Using co-transfection studies, we showed that the NRSE of the MOR promoter is functional in NRSF-positive cells (NS20Y and HeLa) but not in NRSF-negative cells (PC12).