+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Primary afferent neurons intrinsic to the guinea-pig intestine, like primary afferent neurons of spinal and cranial sensory ganglia, bind the lectin, IB4



Primary afferent neurons intrinsic to the guinea-pig intestine, like primary afferent neurons of spinal and cranial sensory ganglia, bind the lectin, IB4



Cell and Tissue Research 321(2): 151-157



The plant lectin, IB4, binds to the surfaces of primary afferent neurons of the dorsal root and trigeminal ganglia and is documented to be selective for nociceptive neurons. Physiological data suggest that the intrinsic primary afferent neurons within the intestine are also nociceptors. In this study, we have compared IB4 binding to each of these neuron types in the guinea-pig. The only neurons in the intestine to be readily revealed by IB4 binding have Dogiel-type-II morphology; these neurons have been previously identified as intrinsic primary afferent neurons. Most of the neurons that are IB4-positive in the myenteric plexus are calbindin-immunoreactive, whereas those in the submucosal ganglia are immunoreactive for NeuN. The neurons that bind IB4 strongly have a similar appearance in enteric, dorsal root and trigeminal ganglia. Binding is to the cell surface, to the first part of axons and to cytoplasmic organelles. A low level of binding was found in the extracellular matrix. A few other neurons in all ganglia exhibit faint staining with IB4. Strongly reactive neurons are absent from the gastric corpus. Thus, IB4 binding reveals primary afferent neurons with similar morphologies, patterns of binding and physiological roles in enteric, dorsal root and trigeminal ganglia.

(PDF emailed within 0-6 h: $19.90)

Accession: 012449047

Download citation: RISBibTeXText

PMID: 15912404

DOI: 10.1007/s00441-005-1129-1


Related references

Immunohistochemical localisation of cholinergic markers in putative intrinsic primary afferent neurons of the guinea-pig small intestine. Cell & Tissue Research. 29(1): 35-43,., 1998

The immunohistochemical localization of cholinergic markers in putative intrinsic primary afferent neurons of the guinea-pig small intestine. Society for Neuroscience Abstracts 24(1-2): 1335, 1998

Cranial sensory ganglia neurons require intrinsic N-cadherin function for guidance of afferent fibers to their final targets. Neuroscience 159(3): 1175-1184, 2009

Intrinsic primary afferent neurons of the intestine. Progress in Neurobiology 54(1): 1-18, 1998

Inputs from intrinsic primary afferent neurons to nitric oxide synthase-immunoreactive neurons in the myenteric plexus of guinea pig ileum. Cell & Tissue Research 299(1): 1-8, 2000

P2X receptors mediate synaptic transmission from descending interneurons, but not from intrinsic primary afferent neurons, to inhibitory motor neurons in the guinea-pig ileum. Gastroenterology 118(4 Suppl 2 Part 1): AGA A877, 2000

Intrinsic primary afferent neurons and nerve circuits within the intestine. Progress in Neurobiology 72(2): 143-164, 2004

Substance P as an excitatory transmitter of primary afferent neurons in guinea-pig sympathetic ganglia. Neuroscience 7(9): 2025-2037, 1982

Carbonic anhydrase enzyme histochemistry of cranial nerve primary sensory afferent neurons in the rat. Histochemistry 88(2): 151-154, 1988

Identification of enteric primary afferent neurons in the guinea-pig small intestine. Gastroenterology 122(4 Suppl 1): A 91-A 92, 2002

FlCRhR/cyclic AMP signaling in myenteric ganglia and calbindin-D28 intrinsic primary afferent neurons involves adenylyl cyclases I, III and IV. Brain Research 826(2): 253-269, May 1, 1999

5-HT activates intestinal intrinsic primary afferent neurons via 5-HT3 receptors in the guinea pig myenteric plexus. Society for Neuroscience Abstracts 24(1-2): 879, 1998

Does defensin NP-1 influence the excitability of the primary afferent neurons of the guinea pig small intestine?. Doklady Biological Sciences 401: 100-103, 2005

Spinal tachykinin NK-1 receptors modulate the afferent arm of the micturition reflex through capsaicin-sensitive primary afferent neurons. Journal of the Autonomic Nervous System 43(SUPPL ): 90, 1993

Populations of afferent neurons in the sensory ganglia detected using lectin from Laburnum anagyroides. Morfologiia 124(6): 38-40, 2004