+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Proinsulin expression by Hassall's corpuscles in the mouse thymus

Proinsulin expression by Hassall's corpuscles in the mouse thymus

Diabetes 53(2): 354-359

The thymus expresses proinsulin, among many other tissue-specific antigens, and the inheritance of genetically determined low thymic proinsulin expression has been associated with impaired proinsulin-specific autoreactive T-cell tolerance and type 1 diabetes susceptibility. The cellular and molecular biology of proinsulin expression in the thymus remains unknown, and contradictory reports exist regarding the identity of proinsulin-producing cells. Using knock-in mice expressing beta-galactosidase (beta-Gal) under the control of an endogenous insulin promoter, we found that thymic proinsulin and beta-Gal transcripts were detectable at high levels in purified thymic epithelial cells. Immunohistochemical analysis of beta-Gal activity showed that most proinsulin expression can be accounted for by rare medullary epithelial cells of the Hassall's corpuscles. Moreover, flow cytometry analyses of beta-Gal-positive cells showed that only 1-3% of all epithelial cells express proinsulin, and this technique will now provide us with a method for isolating the proinsulin-producing cells in mouse thymus.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 012456893

Download citation: RISBibTeXText

PMID: 14747285

DOI: 10.2337/diabetes.53.2.354

Related references

The fine structure of Hassall's corpuscles and reticular cells in the mouse thymus. Acta Medica et Biologica 14(3): 107-114, 1966

Effect of x-rays on the mouse thymus gland. 3. Localization of acid phosphatases at the level of Hassall's corpuscles. Rivista di Biologia 61(2): 273-292, 1968

Expression of the janus kinases: Signal transducers and activators of transcription pathway in Hassall's corpuscles of the human thymus. Histochemistry & Cell Biology 113(6): 427-431, 2000

Hassall's corpuscles in chelonian thymus. Current Science (Bangalore): 495: 202-203, 1980

Immunoglobulins in Hassall's corpuscles of the human thymus. Journal of Anatomy 168: 185-197, 1990

Adrenoreceptor properties of Hassall's concentric corpuscles in the thymus. Bulletin of Experimental Biology and Medicine 73(6): 735-736, 1972

Polymorphism of Hassall's corpuscles in thymus of human fetuses. International Journal of Applied and Basic Medical Research 2(1): 7, 2012

Histogenesis and degeneration of Hassall's corpuscles in thymus of buffalo. Indian Journal of Animal Sciences 79(9): 880-882, 2009

Similarities between Hassall's corpuscles of the human thymus and the epidermis. Cell and Tissue Research 151(3): 347-368, 1974

Destruction of Hassall's corpuscles by macrophages in the sheep thymus. Cell and Tissue Research 217(1): 49-54, 1981

Variety Of Central Cells In Hassall's Corpuscles Of The Human Thymus. Zeitschrift für Mikroskopisch-Anatomische Forschung 71: 10-23, 1964

The formation of thymus cysts and Hassall's corpuscles in model experiments. Acta Morphologica Academiae Scientiarum Hungaricae 11: 403-414, 1962

Aged human thymus hassall's corpuscles are immunoreactive for IGF-I and IGF-I receptor. Anatomical Record 292(7): 960-965, 2009

Development and degeneration of the second type of Hassall's corpuscles in the thymus of guinea pig. Anatomical Record 142: 195-203, 1962

Hassall's corpuscles: a measure of activity in the thymus during involution and reconstitution. Immunology 15(4): 561-570, 1968