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Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells



Prostaglandin E2 stimulates angiogenesis by activating the nitric oxide/cGMP pathway in human umbilical vein endothelial cells



Experimental & Molecular Medicine 37(6): 588-600



Prostaglandin E-2 (PGE(2)), a major product of cyclooxygenase, has been implicated in modulating angiogenesis, vascular function, and inflammatory processes, but the underlying mechanism is not clearly elucidated, We here investigated the molecular mechanism by which PGE(2) regulates angiogenesis. Treatment of human umbilical vein endothelial cells (HUVEC) with PGE(2) increased angiogenesis. PGE(2) increased phosphorylation of Akt and endothelial nitric oxide synthase (eNOS), eNOS activity, and nitric oxide (NO) production by the activation of cAMP-dependent protein kinase (PKA) and phosphatidylinositol 3-kinase (P13K). Dibutyryl cAMP (DB-cAMP) mimicked the role of PGE(2) in angiogenesis and the signaling pathway, suggesting that cAMP is a down-stream mediator of PGE(2). Furthermore, PGE(2) increased endothelial cell sprouting from normal murine aortic segments, but not from eNOS-deficient ones, on Matrigel. The angiogenic effects of PGE(2) were inhibited by the inhibitors of PKA, P13K, eNOS, and soluble guanylate cyclase, but not by phospholipase C inhibitor. These results clearly show that PGE2 increased angiogenesis by activating the NO/cGMP signaling pathway through PKA/Pl3K/Akt-dependent increase in eNOS activity.

Accession: 012460105

Download citation: RISBibTeXText

PMID: 16391520

DOI: 10.1038/emm.2005.72


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