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Quantitative biofilm assay using a microtiter plate to screen for enteroaggregative Escherichia coli



Quantitative biofilm assay using a microtiter plate to screen for enteroaggregative Escherichia coli



American Journal of Tropical Medicine and Hygiene 71(5): 687-690



The gold standard for identification of Enteroaggregative Escherichia coli (EAEC) remains the HEp-2 cell adherence test, which is time-consuming and requires specialized facilities. We evaluated the usefulness of a quantitative biofilm assay to screen for EAEC from a total of 1,042 E. coli strains from children with diarrhea. Bacteria were incubated overnight in high-glucose Dulbecco's modified Eagle's medium using a polystyrene microtiter plate.

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Accession: 012474817

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PMID: 15569806


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