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Rapid and sensitive method for evaluation of radical-scavenging activity using peroxyl radicals derived from 2,2 '-Azobis (2-amidinopropane) dihydrochloride combined with luminol chemiluminescence

Kitao, S.; Fujii, K.; Teramoto, M.; Harada, K.; Ando, M.; Tamura, Y.

FOOD SCIENCE AND TECHNOLOGY RESEARCH 11(3): 318-323

2005

ISSN/ISBN: 1344-6606
DOI: 10.3136/fstr.11.318
Accession: 012481833
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A rapid and sensitive method for evaluation of the radical-scavenging activity, which uses 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) as a radical generator in combination with a luminol chemiluminescence (CL) assay, was examined. The radical-scavenging activities of various analytical-grade compounds were measured by monitoring the decrease in CL. In this method (AAPH-CL), compounds that show lower CL values have stronger radical-scavenging activity.

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Related References

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Beauseigneur, F.; Goubern, M.; Chapey, M.F.; Gresti, J.; Vergely, C.; Tsoko, M.; Demarquoy, J.; Rochette, L.; Clouet, P. 1996: F1F0-ATPase, early target of the radical initiator 2,2'-azobis-(2-amidinopropane) dihydrochloride in rat liver mitochondria in vitro Biochemical Journal 320: 571-576
Sasakii, K.; Kimura, K.; Kitaguchi, Y.; Onoue, T.; Ogura, H.; Aoyagi, Y. 2001: Changes in ascorbic acid content in primary cultured rat hepatocytes exposed to 2,2'-azobis (2-amidinopropane) dihydrochloride, a radical initiator Journal of Veterinary Medical Science 63(8): 925-927
Kanno, T.; Utsumi, T.; Ide, A.; Takehara, Y.; Saibara, T.; Akiyama, J.; Yoshioka, T.; Utsumi, K. 1994: Dysfunction of mouse liver mitochondria induced by 2,2'-azobis-(2-amidinopropane) dihydrochloride, a radical initiator, in vitro and in vivo Free Radical Research 21(4): 223-234
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Vanzani, P.; Rossetto, M.; De Marco, V.; Rigo, A.; Scarpa, M. 2011: Efficiency and capacity of antioxidant rich foods in trapping peroxyl radicals: A full evaluation of radical scavenging activity Food Research International 44(1): 269-275
Betigeri, S.; Thakur, A.; Raghavan, K. 2005: Use of 2,2'-azobis(2-amidinopropane) dihydrochloride as a reagent tool for evaluation of oxidative stability of drugs Pharmaceutical Research 22(2): 310-317
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Henkow, L.; Strid, A.; Berglund, T.; Rydstrom, J.; Ohlsson, A.B. 1996: Alteration of gene expression in Pisum sativum tissue cultures caused by the free radical-generating agent 2,2'-azobis (2-amidinopropane) dihydrochloride Physiologia Plantarum 96(1): 6-12