EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Rapid quantification of the toxic alga Prymnesium parvum in natural samples by use of a specific monoclonal antibody and solid-phase cytometry


Rapid quantification of the toxic alga Prymnesium parvum in natural samples by use of a specific monoclonal antibody and solid-phase cytometry



Applied and Environmental Microbiology 72(1): 860-868



ISSN/ISBN: 0099-2240

PMID: 16391128

DOI: 10.1128/aem.72.1.860-868.2006

The increasing incidence of harmful algal blooms around the world and their associated health and economic effects require the development of methods to rapidly and accurately detect and enumerate the target species. Here we describe use of a solid-phase cytometer to detect and enumerate the toxic alga Prymnesium parvum in natural samples, using a specific monoclonal antibody and indirect immunofluorescence. The immunoglobulin G antibody 16E4 exhibited narrow specificity in that it recognized several P. parvum strains and a Prymnesium nemamethecum strain but it did not cross-react with P. parvum strains from Scandinavia or any other algal strains, including species of the closely related genus Chrysochromulina. Prymnesium sp. cells labeled with 16E4 were readily detected by the solid-phase cytometer because of the large fluorescence signal and the signal/noise ratio. Immunofluorescence detection and enumeration of cultured P. parvum cells preserved with different fixatives showed that the highest cell counts were obtained when cells were fixed with either glutaraldehyde or formaldehyde plus the cell protectant Pluronic F-68, whereas the use of formaldehyde alone resulted in significantly lower counts. Immunofluorescence labeling and analysis with the solid-phase cytometer of fixed natural samples from a bloom of P. parvum occurring in Lake Colorado in Texas gave cell counts that were close to those obtained by the traditional method of counting using light microscopy. These results show that a solid-phase cytometer can be used to rapidly enumerate natural P. parvum cells and that it could be used to detect other toxic algae, with an appropriate antibody or DNA probe.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 012483115

Download citation: RISBibTeXText

Related references

Automated detection and enumeration for toxic algae by solid-phase cytometry and the introduction of a new probe for Prymnesium parvum (Haptophyta: Prymnesiophyceae). Journal of Plankton Research 28(7): 643-657, 2006

Rapid detection and quantification of Aspergillus fumigatus in environmental air samples using solid-phase cytometry. Environmental Science and Technology 43(9): 3233-3239, 2009

Rapid quantification of viable fungi in hospital environments: analysis of air and surface samples using solid-phase cytometry. Journal of Hospital Infection 83(2): 122-126, 2013

Occurrence of toxic alga prymnesium parvum in water bodies of the south ukrainian ssr. Ukrayins'kyi Botanichnyi Zhurnal 30(5): 631-635, 1973

Prymnesins: toxic metabolites of the golden alga, Prymnesium parvum Carter (Haptophyta). Marine Drugs 8(3): 678-704, 2010

Identification of toxic fatty acid amides isolated from the harmful alga Prymnesium parvum carter. Harmful Algae 20: 111-116, 2012

Identification of Toxic Fatty Acid Amides Isolated from the Harmful Alga Prymnesium parvum Carter. Harmful Algae 20(none), 2012

Nutrient Manipulation to Control the Toxic Alga Prymnesium parvum : Verification of Treatments and Resolution of the Issue of Elevated pH. North American Journal of Aquaculture 73(2): 141-152, 2011

Rapid and sensitive quantification of Vibrio cholerae and Vibrio mimicus cells in water samples by use of catalyzed reporter deposition fluorescence in situ hybridization combined with solid-phase cytometry. Applied and Environmental Microbiology 78(20): 7369-7375, 2012

Retrospective analysis of associations between water quality and toxic blooms of golden alga (Prymnesium parvum) in Texas reservoirs: Implications for understanding dispersal mechanisms and impacts of climate change. Harmful Algae 33: 1-11, 2014

A rapid and highly sensitive solid-phase enzyme immunoassay specific for human fibronectin using a characterized monoclonal antibody. Journal of Immunological Methods 72(1): 145-156, 1984

Solid phase cytometry allows rapid in situ quantification of human papilloma virus infection in biopsy material. Cytometry Part A 29(4): 292-297, 1997

Solid phase cytometry allows rapid in situ quantification of human papilloma virus infection in biopsy material. Cytometry 29(4): 292-297, 1997

Rapid and direct quantification of viable Candida species in whole blood by use of immunomagnetic separation and solid-phase cytometry. Journal of Clinical Microbiology 48(4): 1126-1131, 2010

Biological activities of prymnesin-2 isolated from a red tide alga Prymnesium parvum. Natural Toxins 6(1): 35-41, 1998