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Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus



Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus



Emerging Infectious Diseases 10(2): 311-316



A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

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Accession: 012486200

Download citation: RISBibTeXText

PMID: 15030703

DOI: 10.3201/eid1002.030759


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