+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements

Selecting the right fluorophores and flow cytometer for fluorescence resonance energy transfer measurements

Cytometry. Part A 65(2): 148-157

Background: Fluorescence resonance energy transfer applied in flow cytometry (FCET) is an excellent toot for determining supramolecular organization of biomolecules at the cell surface or inside the cell.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 012541401

Download citation: RISBibTeXText

PMID: 15825180

DOI: 10.1002/cyto.a.20142

Related references

Long wavelength fluorophores and cell-by-cell correction for autofluorescence significantly improves the accuracy of flow cytometric energy transfer measurements on a dual-laser benchtop flow cytometer. Cytometry 48(3): 124-135, 2002

A high-throughput direct fluorescence resonance energy transfer-based assay for analyzing apoptotic proteases using flow cytometry and fluorescence lifetime measurements. Analytical Biochemistry 491: 10-17, 2015

Characterization of Fluorescence Resonance Energy Transfer between pairs of fluorophores using a RNA ruler. Biophysical Journal 86(1): 605a-606a, 2004

Extending Förster resonance energy transfer measurements beyond 100 Å using common organic fluorophores: enhanced transfer in the presence of multiple acceptors. Journal of Biomedical Optics 17(1): 011006, 2012

Spectra and lifetimes of fluorescence resonance energy transfer fluorophores under two-photon excitation. Scanning 25(3): 116-120, 2003

Transition-metal-induced fluorescence resonance energy transfer in a cryptand derivatized with two different fluorophores. Inorganic Chemistry 46(19): 8051-8058, 2007

Non-natural amino acid fluorophores for one- and two-step fluorescence resonance energy transfer applications. Analytical Biochemistry 399(2): 182-189, 2010

Novel calibration method for flow cytometric fluorescence resonance energy transfer measurements between visible fluorescent proteins. Cytometry. Part a 67(2): 86-96, 2005

Chip-based protease assay using fluorescence resonance energy transfer between quantum dots and fluorophores. BioChip Journal 1(4): 228-233, 2007

ATP-induced transconformation of myosin revealed by determining three-dimensional positions of fluorophores from fluorescence energy transfer measurements. Journal of Structural Biology 132(1): 6, 2000

Development of homogeneous binding assays based on fluorescence resonance energy transfer between quantum dots and Alexa Fluor fluorophores. Analytical Biochemistry 357(1): 68-76, 2006

Fluorescence resonance energy transfer and arrangements of fluorophores in integrated coumarin/cyanine systems within solid-state two-dimensional nanospace. Journal of Photochemistry and Photobiology A: Chemistry 225(1): 125-134, 2011

Improving flow cytometric energy transfer measurements on a commercial flow cytometer using cell-by-cell correction for autofluorescence. Cytometry Suppl. (11): 125, 2002

Use of tandem cuvettes to determine whether radiative (trivial) energy transfer can contaminate steady-state measurements of fluorescence resonance energy transfer. Analytical Biochemistry 230(1): 187-191, 1995

Novel diode laser-compatible fluorophores and their application to single molecule detection, protein labeling and fluorescence resonance energy transfer immunoassay. Photochemistry & Photobiology 74(2): 237-245, 2001