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Severe acute respiratory syndrome coronavirus persistence in Vero cells



Severe acute respiratory syndrome coronavirus persistence in Vero cells



Chinese Medical Journal 118(6): 451-459



Background Several coronaviruses establish persistent infections in vitro and in vivo, however it is unknown whether persistence is a feature of the severe acute respiratory syndorme coronavirus (SARS-CoV) life cycle. This study was conduced to investigate viral persistence.Methods We inoculated confluent monolayers of Vero cells with SARS-CoV at a multiplicity of infection of 0.1 TCID50 and passaged the remaining cells every 4 to 8 days for a total of 11 passages.

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Accession: 012550745

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PMID: 15788125


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