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Simultaneous determination of zonisamide, carbamazepine and carbamazepine-10,11-epoxide in infant serum by high-performance liquid chromatography



Simultaneous determination of zonisamide, carbamazepine and carbamazepine-10,11-epoxide in infant serum by high-performance liquid chromatography



Journal of Pharmaceutical and Biomedical Analysis 41(4): 1386-1390



This study developed a simple method for the simultaneous determination of zonisamide (ZNS), carbarnazepine (CBZ) and its active metabolite, carbamazepine-10,11-epoxide (CBZE) in infant serum using reversed-phase high-performance liquid chromatograph (HPLC). The method involves a single-step protein precipitation procedure that uses no solid-phase or liquid-liquid extraction. The HPLC separation was carried out on a Cadenza CD-C 18 column (3 mu m, 4.6 min x 150 mm) with potassium phosphate buffer (pH 4.6; 25 mM)-methanol-acetonitrile (65:20:15 (v/v/v)) as a mobile phase at a 1.0 ml/min flow rate: ZNS was detectable using a UV detector at 235 nm, and both CBZ and CBZE were at 215 nm. The quantification limits were established in accordance with each therapeutic range at 2.5 mu g/ml for ZNS, 0.5 mu g/ml for CBZ, and 0.25 mu g/ml for CBZE. The respective coefficients of variation were 1.3-6.0% and 2.2-7.7% for the intra- and inter-assay. (c) 2006 Elsevier B.V. All rights reserved.

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Accession: 012559781

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PMID: 16597494

DOI: 10.1016/j.jpba.2006.02.044



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