Single strand confirmation polymorphism (SSCP) detection in exon I of the alpha-lactalbumin gene of Indian Jamunapari milk goats (Capra hircus)

Kumar, D.; Gupta, N.; Ahlawat, S.P.S.; Satyanarayana, R.; Sunder, S.; Gupta, S.C.

Genetics and Molecular Biology 29(2): 287-289

2006


ISSN/ISBN: 1415-4757
Accession: 012560955

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Abstract
The genetic diversity of Jamunapari goats (Capra hircus) was investigated using an optimized non-radioactive polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method to detect a-lactalbumin polymorphism in a sample of 50 goats. Our data show that PCR-SSCP is an appropriate tool for evaluating genetic variability in Jamunapari goats. Polymorphism was detected in the sample, indicating that Jamunapari goats have high genetic variability at loci, exon I of the alpha-lactalbumin gene. This result opens interesting prospects for future selection programs and conservation strategies. These alpha-lactalbumin variants can be sequenced and screened in the population to develop single nucleotide polymorphism (SNP) markers for association studies and marker assisted selection.